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本文应用细胞膜放射标记和离子交换层析法测定巨噬细胞（ＭФ）内肌醇－１，４，５－三磷酸（ＩＰ３）、用Ｃａ￣（２＋）指示剂的分光光谱法测定ＭФ内Ｃａ￣（２＋）浓度（［Ｃａ￣（２＋）］）、用ＡＰＡＡＰ桥联酶标法检测ＭФ表面Ｉａ抗原的表达，研究去甲肾上腺素（ＮＥ）对大鼠腹腔的ＭФ内ＩＰ３、［Ｃａ￣（２＋）］ｉ和ＭФ表面ｌａ抗原表达的影响。结果显示ＮＥ（１０￣（－８）ｍｏｌ／Ｌ）可显著增高ＭФ中ＩＰ＿３含量（４４２±２２ｃｐｍ／１０￣６ｃｅｌｌｓ，对照组１０２±８ｃｐｍ／１０￣６ｃｅｌｌｓ，Ｐ＜０．０１）；ＮＥ可使ＭФ内［Ｃａ￣（２＋）］ｉ显著增高（３２２±７８ｎｍｏｌ／Ｌ，对照组９７±１７ｎｍｏｌ／Ｌ，Ｐ＜０．０１）；ＮＥ可显著增高ＭФ表面Ｉ－Ａ、Ｉ－Ｅ抗原的表达（６４±８％、５８±６％，对照组５０±３％，４４±４％，Ｐ＜０．０１）。提示神经递质ＮＥ促进ＭФ表面Ｉａ抗原表达的作用可能是通过第二信使ＩＰ＿３和Ｃａ￣（２＋）介导的。
In this study, inositol-1,4,5-triphosphate (IP3) in macrophage (MΦ) was determined by radioimmunoassay and ion exchange chromatography. The content of intracellular Ca ([Ca ~ (2+)]). The expression of Ia antigen on MΦ surface was detected by APAPAP-linked enzyme-linked enzyme-linked immunosorbent assay to study the effect of norepinephrine (NE) (2 +)] i and MΦ surface la antigen expression. The results showed that NE (10 ~ (-8) mol / L) significantly increased the IP_3 content in MФ (442 ± 22cpm / 10 ~ 6cells, control group 102 ± 8cpm / 10 ~ 6cells, P <0.01) The intramyocardial [Ca¯ (2 +)] i was significantly increased in MФ group (322 ± 78nmol / L, 97 ± 17nmol / L in control group, P <0.01); NE significantly increased the expression of I-A and I- (64 ± 8%, 58 ± 6%, control group 50 ± 3%, 44 ± 4%, P <0.01). It suggested that the neurotransmitter NE could promote the expression of Ia antigen on MΦ surface through the second messenger IP_3 and Ca ~ (2+).