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将１０％福尔马林１．０～１．５ｍｌ经尿道注入大鼠膀胱，于刺激后的０．５，１，２，８，２４，４８ｈ取材，进行兔疫组化（ＡＢＣ法）反应，观察脊髓腰骶段后连合核区的ＳＰ样纤维和终末的分布，并随机抽样进行图像分析、另设对照组，向膀胱内注入生理盐水，０．５，２．０ｈ后取材。结果显示：福尔马林刺激后，腰骶髓后连合核区ＳＰ样阳性纤维和终末与对照组相比，在ｌｈ内明显减少，而在２ｈ时则呈增加趋势，８ｈ又恢复正常。同时本文以同样动物模型，实验组分别于刺激后１，３，８，１２，２４，４８ｈ和５ｄ取材，对照组于刺激后２４ｈ取材，进行原位杂交组化反应，观察腰骶髓背根神经节内ＳＰｍＲＮＡ阳性神经元的数量。结果显示：福尔马林刺激后３ｈ即出现ＳＰｍＲＮＡ表达神经元数量的明显增加，２４ｈ达到高峰，并在４８ｈ以后开始下降，到５ｄ后回落到正常水平。
10% formalin 1.0 ~ 1.5ml transurethral injection of the bladder in rats, 0.5,1,2,8,24,48 h after stimulation were drawn for immunohistochemistry (ABC method) reaction The distribution of SP-like fibers and terminals in the posterior commissural nucleus of the lumbosacral spinal cord was observed. Randomly sampled for image analysis. Another control group was injected with saline into the bladder for 0.5 and 2.0 hours. The results showed that after formalin stimulation, SP positive fibers and terminals in lumbosacral dorsal commissural nucleus were significantly decreased in 1 h and increased in 2 h, and returned to normal in 8 h . At the same time, the same animal model was used in this study. The experimental groups were harvested at 1, 3, 8, 12, 24, 48h and 5d after stimulation respectively. The control group was harvested at 24h after stimulation and the in situ hybridization was performed. Number of SPmRNA positive neurons in the ganglion. The results showed that the number of SPmRNA neurons increased significantly at 3h after formalin stimulation, peaked at 24h, decreased after 48h, and returned to normal after 5d.