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目的观察江苏地产浒苔多糖对四氧嘧啶诱导糖尿病小鼠的影响。方法腹腔注射四氧嘧啶建立糖尿病小鼠模型,将小鼠随机分为空白组、模型组、参芪降糖颗粒(500mg/kg)、浒苔多糖低(100mg/kg)、中(200mg/kg)、高(400mg/kg)剂量组,连续灌胃给药28d,观察给药期间动物体质量、进食和饮水量的变化。于给药28d后测定各组小鼠空腹血糖值、口服糖耐量、糖化血清白蛋白以及血清胰岛素的含量。小鼠处死后,剖取胰腺组织进行病理学观察,测定胰岛数和胰岛面积。结果与空白模型组相比,中、高剂量组浒苔多糖能显著降低糖尿病小鼠空腹血糖水平(P<0.01),血糖曲线下面积(P<0.01),显著增多胰岛数目(P<0.01),增大胰岛面积(P<0.01),升高血清胰岛素含量(P<0.05~0.01),且浒苔多糖高剂量组能显著降低模型小鼠糖化白蛋白的浓度(P<0.05)。结论江苏地产浒苔多糖在200~400mg/kg剂量范围内能降低四氧嘧啶诱导糖尿病小鼠的血糖,改善模型小鼠的耐糖力,缓解胰岛病变程度。该结果将为以江苏地产浒苔多糖为原料的功能性保健品的开发提供理论依据。
Objective To observe the effects of Polysaccharides from Enteromorpha prolifera of Jiangsu Province on alloxan-induced diabetic mice. Methods The diabetic mice model was established by intraperitoneal injection of alloxan. The mice were randomly divided into blank group, model group, Shenqi Jiangtang Granule (500mg / kg), Enteromorpha polysaccharides low (100mg / kg) ), High (400mg / kg) dose group, continuous gavage for 28 days to observe the change of animal body weight, food intake and water intake during the administration. The fasting blood glucose, oral glucose tolerance, glycosylated serum albumin and serum insulin were determined in all groups after 28 days of administration. After the mice were sacrificed, the pancreatic tissues were dissected for pathological observation, and the number of islets and islet area were measured. Results Compared with the blank control group, Enteromorpha polysaccharides could significantly decrease the fasting blood glucose (P <0.01), the area under the blood glucose curve (P <0.01) and the number of islets (P <0.01) , Increased islet area (P <0.01), increased serum insulin (P <0.05 ~ 0.01), and Enteromorpha polysaccharide high dose group could significantly reduce the concentration of glycosylated albumin (P <0.05). Conclusion Polysaccharides of Enteromorpha prolifera in Jiangsu Province can reduce the blood sugar of alloxan-induced diabetic mice in the range of 200-400mg / kg, improve the glucose tolerance and alleviate the degree of pancreatic islet in diabetic mice. The results will provide a theoretical basis for the development of functional health products made from Jiangsu Enteromorpha Polysaccharides.