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目的 :观察活血生肌类中药对骨骼肌钝挫伤后修复过程中IIb型肌球蛋白重链 (myosinheavychain ;MHC)及Ⅰ、Ⅲ型胶原 (Collagen -Ⅰ和Collagen -Ⅲ )mRNA表达的影响。方法 :采用打击装置造成 12 6只大鼠后肢肌群钝挫伤 ,用半定量逆转录 -聚合酶链式反应 (semi -quantitativeRT-PCR)分析mRNA的表达。结果 :(1)急性钝挫伤后 ,各组大鼠骨骼肌IIb型MHC及Ⅰ、Ⅲ型胶原的mRNA表达量均显著增高 (P <0 .0 1)。 (2 )修复过程中 ,注射活血生肌类中药治疗组大鼠IIb型MHCmRNA表达水平的升幅显著高于生理盐水对照组 (P <0 .0 1) ,但低于注射IGF -I治疗组 (P <0 .0 5 )。 (3)修复过程中 ,注射活血生肌类中药治疗组大鼠Ⅰ型与Ⅲ型胶原mRNA的表达水平显著低于生理盐水对照组 (P <0 .0 1) ,与注射IGF -Ⅰ治疗组相比无显著差异 (P <0 .0 5 )。结论 :急性骨骼肌钝挫伤修复过程中 ,注射活血生肌类中药能有效地促进Ⅱb型MHCmRNA表达 ,减少Ⅰ、Ⅲ型胶原mRNA表达 ,并促使Ⅰ型胶原mRNA水平迅速恢复到正常水平
OBJECTIVE: To observe the effect of Huoxue Shengji Chinese Herbs on mRNA expression of type IIb myosin heavy chain (MHC) and type I and type III collagen (Collagen-I and Collagen-III) during the repair of skeletal muscle contusion. Methods: The contusion of the hindlimb muscles in 12 6 rats was blunted. The expression of mRNA was analyzed by semiquantitative RT-PCR (semi-quantitative reverse transcriptase-polymerase chain reaction). Results: (1) After acute blunt contusion, mRNA expression of type IIb MHC and type I and type III collagen in skeletal muscle of rats in each group were significantly increased (P <0.01). (2) During the repair process, the expression of MHCmRNA in type IIb in rats injected with Huoxue Shengjiu was significantly higher than that in saline control group (P <0.01), but lower than that in IGF-I treated group P <0. 05). (3) During the repair process, the expression of type I and type III collagen mRNA in rats treated with injection of Chinese herbal medicine of activating blood sarcopenia was significantly lower than that of saline control group (P <0.01) There was no significant difference (P <0.05). Conclusion: During the repair of acute skeletal muscle contusion, injection of Huoxue Shengjiu can effectively promote the expression of type Ⅱ b MHC mRNA, decrease the mRNA expression of type Ⅰ and type Ⅲ collagen, and promote the rapid recovery of type Ⅰ collagen mRNA to normal level