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目的:探讨TA2小鼠三阴性乳腺癌中乙醛脱氢酶1~+(ALDH1~+)和CD133~+表型的乳腺癌干样细胞在上皮间充质转化(EMT)发生中的作用,及对其生物学行为的影响。方法:使用流式细胞术分析TA2小鼠三阴性乳腺癌组织中ALDH1及CD133的表达量并分选出具有ALDH1~+、ALDH1-、CD133~+、CD133-表型的乳腺癌细胞,将其分别接种于TA2小鼠,根据细胞表型不同设置为ALDH1~+、ALDH1-,CD133~+、CD133-组,观察肿瘤生长情况,并制成组织切片,行三阴性乳腺癌中乳腺癌干细胞表面标记物ALDH1、CD133与EMT相关蛋白Twist1、E-cadherin和VE-cadherin的免疫组织化学检测,分析其表达差异。结果:乳腺癌干细胞标记物ALDH1及CD133在TA2小鼠三阴性乳腺癌中表达率分别为31.2%和6.5%。ALDH1~+、CD133~+组肿瘤生成能力明显强于ALDH1-、CD133-组。免疫组织化学结果显示ALDH1、Twist1、VE-cadherin在ALDH1~+组的表达明显高于ALDH1-组(均P<0.05),E-cadherin在ALDH1~+组的表达低于ALDH1-组(P<0.05)。CD133、Twist1、VE-cadherin在CD133~+组的表达明显高于CD133-组(均P<0.05),Ecadherin在CD133~+组的表达低于CD133-组(P<0.05)。结论:TA2小鼠三阴性乳腺癌中ALDH1~+和CD133~+表型的乳腺癌干样细胞可影响EMT相关蛋白的表达,并促进三阴性乳腺癌的形成。
Objective: To investigate the role of ALDH1 ~ + and CD133 ~ + breast cancer stem-like cells in the development of epithelial mesenchymal transition (EMT) in TA2 mice with triple-negative breast cancer, And its impact on biological behavior. Methods: The expression of ALDH1 and CD133 in TA2 mouse triple negative breast cancer tissues was analyzed by flow cytometry and the breast cancer cells with ALDH1 ~ +, ALDH1-, CD133 ~ + and CD133- The cells were inoculated into TA2 mice respectively and ALDH1 ~ +, ALDH1-, CD133 ~ + and CD133- groups were set according to their cell phenotypes. Tumor growth was observed and tissue sections were made. The surface of breast cancer stem cells in triple negative breast cancer The expression of ALDH1, CD133 and EMT-related proteins Twist1, E-cadherin and VE-cadherin were detected by immunohistochemistry. Results: The expression rates of breast cancer stem cell markers ALDH1 and CD133 in TA2-negative triple-negative breast cancer were 31.2% and 6.5%, respectively. The tumorigenicity of ALDH1 ~ + and CD133 ~ + groups was significantly stronger than that of ALDH1- and CD133- groups. The results of immunohistochemistry showed that the expression of ALDH1, Twist1 and VE-cadherin in ALDH1 ~ + group was significantly higher than that in ALDH1- group (all P <0.05), while the expression of E-cadherin in ALDH1 ~ + group was lower than that in ALDH1- group (P < 0.05). The expressions of CD133, Twist1 and VE-cadherin in CD133 ~ + group were significantly higher than those in CD133- group (all P <0.05). The expression of Ecadherin in CD133 ~ + group was lower than that in CD133- group (P <0.05). CONCLUSIONS: The breast cancer stem-like cells of ALDH1 ~ + and CD133 ~ + phenotypes in TA2 mouse triple negative breast cancer can affect the expression of EMT related proteins and promote the formation of triple negative breast cancer.