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目的观察凝血因子Ⅻ(coagu lation factorⅫ,FⅫ)基因第一外显子(Exon1)46C/T基因多态性在脑血栓患者中的分布频率,探讨FⅫ基因Exon1-46C/T多态性与脑血栓形成的相关性。方法采用聚合酶链-限制性片段长度多态性(PCR-RFLP)及琼脂糖凝胶电泳的方法鉴定89例脑血栓患者和83例健康者46C/T基因型。结果脑血栓患者组的FⅫ基因Exon1-46T/T、C/T、C/C型的分布频率分别为0.417、0.5169、0.0674,正常对照组分别为0.5783、0.3976、0.0241。采用相对危险度(OR)及χ2检验统计学分析,脑血栓组和对照组间Exon1-46(C/T)等位基因频率有显著差异(χ2=4.689,P<0.05)。结论FⅫ基因Exon1-46(C/T)等位基因可能是脑血栓形成相关危险因素之一。
Objective To investigate the frequency of 46 C / T polymorphism of Exon1 gene in patients with cerebral thrombosis and to investigate the relationship between Exon1-46C / T polymorphism of F Ⅻ gene and brain Correlation of thrombosis. Methods The genotypes of 46 C / T in 89 patients with cerebral thromboembolism and 83 healthy controls were identified by polymerase chain-fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis. Results The frequencies of Exon 1-46T / T, C / T and C / C were 0.417, 0.5169 and 0.0674 respectively in the group of patients with cerebral thrombosis and 0.5783, 0.3976 and 0.0241 respectively in the normal control group. The relative risk (OR) and χ2 test statistical analysis, cerebral infarction and control group Exon1-46 (C / T) allele frequency was significantly different (χ2 = 4.689, P <0.05). Conclusion Exon1-46 (C / T) allele of FⅫ gene may be one of the risk factors associated with cerebral thrombosis.