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论述了生物样品低温保存过程中的污染途径及其防治措施。以往人们只着重防止复温过程的污染 ,而认为液氮是无菌的。最近在保存骨髓时发现乙肝病毒交叉污染之后 ,才开始对液氮环境的污染问题进行研究。作者认为 :在各种方法中通过采用双层保存袋可以最有效地达到防止污染的目的。
Discussed the biological samples in the process of low temperature storage of pollution and prevention measures. In the past people only focused on preventing the pollution of the rewarming process, and that liquid nitrogen is sterile. Recently, it was discovered that the cross-contamination of hepatitis B virus after the preservation of bone marrow began to study the pollution of liquid nitrogen environment. The authors argue that the goal of pollution prevention can be most effectively achieved through the use of double-layer preservation bags in a variety of ways.