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背景:研究发现类胆碱物质可增加乙酰胆碱的弥散及终板电流的幅度,对神经肌肉接点功能退化有一定的对抗作用。目的:观察氯化胆碱对制动性骨骼肌萎缩的防治作用及对骨骼肌萎缩大鼠肌肉生成抑制素mRNA表达的影响。方法:将30只雄性SD大鼠随机分为对照组、模型组和治疗组,每组10只。采用可塑性石膏固定模型组和治疗组大鼠右后肢制备肌萎缩模型。治疗组每日灌胃氯化胆碱(150mg/kg),对照组和模型组灌胃等体积蒸馏水。4周后解剖右后肢腓肠肌,检测腓肠肌收缩张力、肌湿质量、蛋白质水平及肌肉生成抑制素mRNA的表达。结果与结论:与对照组比较,模型组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著降低(P<0.05或P<0.01),肌肉生成抑制素mRNA表达显著增高(P<0.01)。与模型组比较,治疗组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著升高(P<0.05),肌肉生成抑制素mRNA表达显著降低(P<0.05)。说明氯化胆碱能够显著提高制动性萎缩骨骼肌的收缩张力、肌湿质量、蛋白质水平,减少肌肉生成抑制素mRNA的表达,从而有效抑制骨骼肌制动性萎缩的发生。
Background: The study found that choline substances can increase the diffusion of acetylcholine and endplate current amplitude, neurodermic junctional degeneration has some antagonistic effect. Objective: To observe the effect of choline chloride on the prevention and treatment of skeletal muscle atrophy and its effect on the expression of myostatin mRNA in skeletal muscle atrophic rats. Methods: Thirty male SD rats were randomly divided into control group, model group and treatment group, with 10 rats in each group. Muscle atrophy model was established by plastic plaster fixation model group and treatment group right hind limbs. The treatment group was given choline chloride (150mg / kg) daily, and the control group and model group were given the same volume of distilled water. After 4 weeks, the right hindlimb gastrocnemius was dissected and the contractile and gastrocnemius muscle muscle mass, protein level and mRNA expression of myostatin were measured. RESULTS AND CONCLUSION: Compared with the control group, the contractile tension, muscle wet weight and protein level in the gastrocnemius muscle of the model group were significantly decreased (P <0.05 or P <0.01), and the mRNA expression of the myostatin significantly increased (P <0.01) . Compared with model group, the contractile tension, muscle wet weight and protein level of gastrocnemius muscle of treatment group were significantly increased (P <0.05), and the expression of myostatin mRNA was significantly decreased (P <0.05). Choline chloride can significantly improve the contraction of the atrophic skeletal muscle contraction, muscle wet weight, protein levels, reduce the expression of myostatin mRNA, thereby effectively inhibiting the occurrence of skeletal muscle brake atrophy.