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目的 探讨补肾益精方含药血清的制备条件及其对体外培养破骨细胞骨吸收功能的影响。方法 取体重 2 70± 2 0g的SD大鼠 ,雌雄各半 ,每组 10只 ,同等条件下制备补肾益精方含药血清和对照血清 ,分别观察不同采血时间、不同喂药时间、不同灌胃剂量和血清添加量对骨吸收陷窝数量的影响。结果 末次灌胃后 1h采血组的骨吸收陷窝数明显少于其他各组 (P <0 0 5 ) ;灌胃 1d和 3d、7d组比较其含药血清的药效无差异 ,而与对照血清差异显著 ;随着灌胃剂量和含药血清添加量的提高 ,其骨吸收陷窝数明显减少。结论 补肾益精方含药血清对破骨细胞骨吸收功能具有明显的抑制作用 ;补肾益精方用于破骨细胞药效观察的大鼠含药血清制备条件为等效剂量连续两次 (间隔 2h)灌胃 ,末次灌胃后1h采血 ,其血清添加浓度为 30 %。
Objective To investigate the preparation conditions of serum containing Bushen Yijing Recipe and its effect on bone resorption of osteoclasts cultured in vitro. Methods SD rats weighing 270±20g were divided into two groups, 10 males and females. The serum containing Bushen Yijing Recipe and the control serum were prepared under the same conditions. The different blood collection time, different feeding time and different irrigation were observed. The effect of stomach dose and serum supplementation on the number of resorption lacunae in bone. Results The number of bone resorption lacunae in the blood collection group was significantly less than that in the other groups (P < 0.05) at the 1h after the last gavage; the efficacy of the drug-containing serum was not different between the 1d and 3d, 7d gavage groups. Serum differences were significant; with the increase in the dose of gastric administration and serum containing drugs, the number of bone resorption lacunae was significantly reduced. Conclusion The serum containing Bushen Yijing Recipe has obvious inhibitory effect on the bone resorption of osteoclasts; the method of Bushen Yijing Recipe for observing the efficacy of osteoclasts in rat serum contains the equivalent dose of two consecutive times (interval 2h) Gavage, blood was collected 1 h after the last gavage, and the serum concentration was 30%.