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本试验目的:用结实率较高的品种间杂种胚为试材,作胚培养条件的试验研究。试图掌握适宜于小麦胚的培养条件与方法,从而为大小麦杂种胚的人工培养奠定基础。材料和方法试材为73(36)-1-9-26×官村1号的 F_1 15天杂种胚作幼胚离体培养。基本培养基(mg/升):T.K 和 white 两种。附加成分:水解乳蛋白(CH)100—300,动力精(KT)1—2,吲哚乙酸(IAA)0.5—1.0。蔗糖浓度8％,琼脂0.8％,PH 值5.8。灭菌方法:幼胚先经70％乙醇表面灭菌2—3秒,转入0.1％氯化汞溶液浸泡5分钟,然后用无菌水冲洗3次。在无菌条件下将果实内幼胚剥离(或切割)下来,直接(不用褓姆法)
The purpose of this experiment: With higher seed setting rate of hybrid embryos as materials, embryo culture conditions of the experimental study. Trying to master the appropriate conditions and methods for the cultivation of wheat germ, which laid the foundation for the cultivation of large and medium-sized hybrid embryos. MATERIALS AND METHODS: F_1 15-day hybrid embryos of 73 (36) -1-9-26 × Guancun 1 were used for in vitro culture. Basic medium (mg / liter): T.K and white two kinds. Additional Ingredients: Hydrolyzed milk protein (CH) 100-300, KT 1-2, Indole Acetic Acid (IAA) 0.5-1.0. 8% sucrose, 0.8% agar, pH 5.8. Sterilization: embryos first 70% ethanol surface sterilization 2-3 seconds, into 0.1% mercuric chloride solution for 5 minutes, then rinse with sterile water 3 times. Aseptically dissect (or cut) the immature embryos in the fruit directly (without using the Lim method)