【摘 要】
：
目的 以Mst1 KO、WASp KO、Mst1-WASp DKO和野生型模型小鼠为研究对象,探究Mst1和WASp共同作用对调节性T细胞(Treg)发育和功能影响及作用机制.方法 使用流式细胞仪检测胸腺和脾脏中T细胞各亚群特别是Treg细胞的比例、数量及其相关分子的表达,并通过构建DKO骨髓嵌合小鼠验证表型变化是否为自发性.结果 与野生型小鼠相比,DKO小鼠胸腺的T细胞各亚群比例无明显变化,而脾脏中T细胞各亚群细胞数均降低,除活化T细胞和Treg的比例升高外,其他各T细胞亚群比例均降低.DKO小鼠脾脏T
【机 构】
：
400014,儿童感染免疫重庆市重点实验室,重庆医科大学附属儿童医院儿研所,国家儿童健康与疾病临床医学研究中心,儿童发育疾病研究教育部重点实验室,儿童发育重大疾病国家国际科技合作基地
论文部分内容阅读
目的 以Mst1 KO、WASp KO、Mst1-WASp DKO和野生型模型小鼠为研究对象,探究Mst1和WASp共同作用对调节性T细胞(Treg)发育和功能影响及作用机制.方法 使用流式细胞仪检测胸腺和脾脏中T细胞各亚群特别是Treg细胞的比例、数量及其相关分子的表达,并通过构建DKO骨髓嵌合小鼠验证表型变化是否为自发性.结果 与野生型小鼠相比,DKO小鼠胸腺的T细胞各亚群比例无明显变化,而脾脏中T细胞各亚群细胞数均降低,除活化T细胞和Treg的比例升高外,其他各T细胞亚群比例均降低.DKO小鼠脾脏Treg中的NRP-1表达减少,CTLA-4的表达增加.骨髓嵌合动物模型中Treg的检测结果与上述一致.结论 Mst1与WASp以细胞固有方式共同影响Treg细胞外周稳态和功能,其机制可能涉及NRP-1和CTLA-4.
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