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目的探讨 ＴＴＶ与病原不明肝炎的关系及 ＴＴＶ能否在肝组织中复制。方法用巢式 ＰＣＲ法对３１例某职校流行的病原不明肝炎患者的血清进行３．２ｋｂＴＴＶ ＤＮＡ扩增， ３０例同地区志愿献血员为对照组，结合肝炎患者的病理改变进行分析；同时用核酸酶保护法对其中７例肝炎患者的肝组织做ＴＴＶ正链的检测。结果在病原不明肝炎患者中，ＴＴＶ的检出率为９６．９％，在志愿献血员中检出率为６０％，两组间ＴＴＶ的检出率差异有显著性。在ＴＴＶ ＤＮＡ阳性的３０例肝炎患者中，尽管大多数肝脏损害轻微，但仍有６．６％有慢性肝炎改变。在７例病原不明肝炎患者的肝组织中用核酸酶保护法均检出ＴＴＶ ＤＮＡ的正链。结论 ＴＴＶ感染可能与该病原不明肝炎的流行有关，ＴＴＶ可以在人肝组织中复制，ＴＴＶ可能导致的肝脏损害轻微，但仍有少数有慢性肝炎发生。
Objective To investigate the relationship between TTV and pathogen-unknown hepatitis and whether TTV can replicate in liver tissue. Methods The nested PCR method was used to amplify the 3.2kb TTV DNA of 31 cases of hepatitis A virus of unknown hepatitis in a vocational school, and 30 cases of volunteer blood donors in the same area as the control group were analyzed with the pathological changes of hepatitis. At the same time, Nuclease protection method in which seven cases of hepatitis patients with liver TTV positive chain detection. Results The detection rate of TTV was 96.9% in patients with unknown pathogen hepatitis and 60% in volunteer blood donors. There was a significant difference in the detection rate of TTV between the two groups. Of the 30 TTV DNA-positive hepatitis patients, 6.6% of them still had chronic hepatitis changes, although most liver damage was mild. The positive strand of TTV DNA was detected by nuclease protection in the liver tissue of 7 patients with pathogen-free hepatitis. Conclusion TTV infection may be related to the epidemic of unidentified hepatitis in this pathogen. TTV can replicate in human liver tissue. TTV may cause slight liver damage, but there are still a few cases of chronic hepatitis.