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目的探讨疏肝健脾方药对非酒精性脂肪性肝炎(NASH)大鼠肝组织核因子(NF)-κB mRNA及蛋白表达的影响。方法采用高脂饲料喂养复制NASH大鼠实验模型,施以造模因素的同时,各用药组大鼠分别灌服疏肝方、健脾方和疏肝健脾合方的高、低剂量进行干预。16 w后,各组大鼠以3%戊巴比妥钠腹腔麻醉,腹主动脉采血,全自动生化分析仪检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量,肝组织TC、TG含量;HE染色和油红O染色观察肝组织病理变化;实时定量PCR法检测肝组织NF-κB mRNA表达水平;Western印迹法检测肝组织NF-κB蛋白表达水平。结果与正常组相比,模型组大鼠肝细胞脂肪变性明显,血脂及肝脂均有不同程度的升高(P<0.05,P<0.01),大鼠肝组织NF-κB mRNA及蛋白表达明显升高(P<0.01);与模型组相比,各用药组血脂和肝组织NF-κB mRNA及蛋白的表达均较模型组显著降低(P<0.05,P<0.01),尤以综合方高剂量及健脾高剂量作用最为显著。结论疏肝健脾方药对高脂饮食诱导的大鼠NASH有较好的防治作用,其机制可能与其下调肝脏NF-κB mRNA及蛋白的表达有关。
Objective To investigate the effect of Shuganjianpi Recipe on expression of nuclear factor (NF) -κB mRNA and protein in liver of nonalcoholic steatohepatitis (NASH) rats. Methods The experimental model of NASH rats was fed with high-fat diet. Simultaneously, high and low doses of Shuganfang, Jianpifang and Shuganjianpi were administered to rats in each treatment group . After 16 w, the rats in each group were anesthetized with 3% sodium pentobarbital, abdominal aorta, and the levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol HDL-C), LDL-C and TC, TG contents in liver tissue were observed. The pathological changes of liver were observed by HE staining and oil red O staining. The expression of NF-κB mRNA in liver tissue was detected by real-time quantitative PCR Western blotting was used to detect the expression of NF-κB in liver tissue. Results Compared with the normal group, the fatty degeneration of hepatic cells in the model group was obvious, and the levels of lipids and hepatic lipids were increased to some extent (P <0.05, P <0.01). The expression of NF-κB mRNA and protein in the liver tissue of the model group was significantly (P <0.01). Compared with the model group, the expressions of NF-κB mRNA and protein in the blood lipids and liver tissues of the treatment groups were significantly lower than those of the model group (P <0.05, P <0.01) Dosage and spleen high dose of the most significant effect. Conclusion Shuganjianpi Decoction has good preventive and therapeutic effects on NASH induced by high-fat diet in rats, which may be related to the down-regulation of NF-κB mRNA and protein expression in the liver.