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目的采用高通量的蛋白质组学技术研究人乳腺癌细胞株MCF7细胞受GSM1800MHz射频电磁场辐照后,其蛋白质表达谱的变化,以探索移动电话信号对细胞正常生理功能的可能影响。方法比吸收率为3.5W/kg时,采用不同时间(1、3、6、12和24h)和辐照模式(间断辐射和连续辐射)的GSM1800MHz射频电磁场处理MCF7细胞后,直接抽提蛋白质,然后进行双向凝胶电泳。凝胶经银染后,使用PDQuest软件分析假辐照组与电磁场辐照组间的差异表达蛋白质斑点。每个实验重复3次。结果凝胶上平均可检测到1100个蛋白斑点。3.5W/kg连续辐射6h未发现差异点,其他暴露情况下均可检测到不同数量的差异蛋白,以3.5W/kg间断辐射3h和连续辐照12h检测的差异蛋白点较多,分别为18个和7个。通过搜索SWISSPROT蛋白数据库,对差异蛋白的类别和功能进行了初步推测,结果表明差异蛋白主要与生物分子的合成和调控、信号转导、DNA损伤修复等功能相关。结论GSM1800MHz射频电磁场对MCF7细胞蛋白质表达谱具有一定程度的影响,且依赖于暴露的强度、时间和模式,影响环节可能涉及多个生物学过程。
OBJECTIVE: To study the changes of protein expression profile of human breast cancer cell line MCF7 after being irradiated by GSM1800MHz RF electromagnetic field with high-throughput proteomics to explore the possible effect of cell phone signal on the normal physiological function of cells. Methods The specific absorption rate of 3.5W / kg, using different time (1,3,6,12 and 24h) and irradiation mode (intermittent radiation and continuous radiation) GSM1800MHz RF electromagnetic field treatment of MCF7 cells directly extracted protein, Then two-dimensional gel electrophoresis. After the gel was silver-stained, PDQuest software was used to analyze the differential expression of protein spots between the sham irradiation group and the electromagnetic field irradiation group. Repeat 3 times for each experiment. As a result, an average of 1,100 protein spots were detected on the gel. 3.5W / kg continuous radiation for 6h did not find differences in the number of different exposures were detected in different numbers of different proteins to 3.5W / kg intermittent radiation 3h and continuous irradiation 12h detection of the protein spots more points were 18 One and seven. By searching the SWISSPROT protein database, we initially speculated on the types and functions of the differentially expressed proteins. The results showed that the differential proteins were mainly related to the functions of biomolecule synthesis and regulation, signal transduction and DNA damage repair. Conclusion GSM1800MHz RF electromagnetic field has a certain degree of influence on the protein expression profile of MCF7 cells and depends on the intensity, timing and mode of exposure, and may influence multiple biological processes.