本文研究了灯盏花素抑制脂质过氧化的作用机制。大鼠脑线粒体脂质过氧化物用硫代巴比妥酸比色法测定。灯盏花素与铁的螯合活性用差示光谱法测定。黄嘌呤黄嘌呤氧化酶（ＸａｎＸＯ）体系产生的超氧阴离子自由基（Ｏ２）及ＦｅＳＯ４Ｈ２Ｏ２体系产生的羟自由基（·ＯＨ）用比色法测定。结果表明：灯盏花素能有效地抑制ＸａｎＸＯ和ＦｅＳＯ４Ｈ２Ｏ２诱导的脑线粒体脂质过氧化反应，其ＩＣ５０分别为９３０１和６２１８μｍｏｌ·Ｌ－１。灯盏花素也能清除ＸａｎＸＯ体系产生的Ｏ２和ＦｅＳＯ４Ｈ２Ｏ２体系产生的·ＯＨ，其ＩＣ５０分别为３２６３和２０２２μｍｏｌ·Ｌ－１。灯盏花素还具有螯合Ｆｅ２＋的活性。由此可见，灯盏花素是在氧自由基与线粒体膜的反应中（１）·ＯＨ的形成（通过与Ｆｅ２＋螯合）（２）脂质过氧化的启动（通过清除Ｏ２和·ＯＨ）两个环节抑制脂质过氧化反应的。清除氧自由基和与Ｆｅ２＋螯合是灯盏花素抑制脑线粒体脂质过氧化的作用机制 This paper studied the mechanism of action of scutellarin on lipid peroxidation inhibition. Rat brain mitochondrial lipid peroxides were determined by thiobarbituric acid colorimetry. The chelating activity of erigeronin and iron was determined by differential spectroscopy. The superoxide anion radical (O2) produced by the xanthine-xanthine oxidase (Xan-XO) system and the hydroxyl radical (·OH) produced by the FeSO4H2O2 system were determined by colorimetry. The results showed that breviscapine could effectively inhibit the lipid peroxidation of brain mitochondria induced by XanXO and FeSO4H2O2 with the IC50 of 9301 and 6218μmol·L-1, respectively. Breviscapine can also remove the OH produced by the O2 and FeSO4H2O2 systems produced by the XanXO system, and its IC50 is 3263 and 2022μmol·L-1, respectively. Breviscapine also has the activity of chelating Fe2+. It can be seen that breviscapine is in the reaction of oxygen radicals and mitochondrial membrane (1) ·OH formation (by chelation with Fe2+) (2) initiation of lipid peroxidation (by scavenging O2 and ·OH) The links inhibit lipid peroxidation. Oxygen free radical scavenging and chelation with Fe2+ are the mechanisms by which breviscapine inhibits lipid peroxidation of brain mitochondria