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哺乳动物生精过程是一个精细的调节过程 ,人们对于这个过程还了解甚少。这主要是由于维持生殖细胞体外培养的必需条件尚未建立 ,睾丸生精细胞可原代培养的时间太短。因此 ,有必要建立能在体外长期传代的睾丸生精细胞株。Hofmann等人用磷酸钙法将猴病毒 40大T抗原 (sv40lt)基因掺入小鼠睾丸细胞建立了永生的睾丸细胞株 ,并利用这些细胞株研究了睾丸特异基因的表达。随后 ,又通过对小鼠睾丸生精细胞共转染sv40lt基因和编码温度敏感p5 3蛋白基因 ,建立了两个能在体外继续分化的生精细胞株。这些睾丸细胞株的建立为精子发生研究提供了一个有用的实验模型。
Mammalian spermatogenesis is a delicate process of regulation, and little is known about this process. This is mainly due to the fact that the necessary conditions for maintaining in vitro culture of germ cells have not yet been established, and the time for primary culture of testicular spermatogenic cells can be too short. Therefore, it is necessary to establish testicular spermatogenic cell lines that can be passaged in vitro in long term. Hofmann et al. Established the immortal testicular cell line by incorporating the 40-mer Sv40lt gene of simian virus into the testicular cells of the mouse by the calcium phosphate method and using these cell lines to study the expression of the testis-specific genes. Subsequently, two spermatogenic cell lines that can continue to differentiate in vitro were established by co-transfecting the sv40lt gene and the gene encoding the temperature-sensitive p5 3 protein in mouse testicular germ cells. The establishment of these testicular cell lines provides a useful experimental model for spermatogenesis research.