论文部分内容阅读
目的 探讨抑癌基因p16在食管鳞癌组织中的蛋白表达与临床、病理变化之间的关系。方法 应用改良SABC免疫组化法 ,对 5 4例手术切除的原发食道鳞癌患者的肿瘤组织蜡块进行p16基因蛋白表达产物的测定。结果 5 4例食管鳞癌病人中p16基因表达缺失 2 1例 ( 38.9% )。p16表达与肿瘤病理分级关系密切 ,p16表达缺失者肿瘤组织分化程度明显低于p16表达阳性者 (P <0 .0 0 0 1)。p16基因表达与区域淋巴结转移也有一定的相关性。结论 原发性食管鳞癌患者的p16基因蛋白表达缺失较常见 ,且与肿瘤组织的分化程度密切相关 ,与淋巴结转移也有一定的关系。利用SABC免疫组化法检则p16基因的表达情况 ,可作为原发性食管鳞癌的辅助诊断手段 ,同时也为选择恰当的治疗方案提供了重要的信息。
Objective To investigate the relationship between the expression of tumor suppressor gene p16 and clinical and pathological changes in esophageal squamous cell carcinoma. Methods The modified SABC immunohistochemical method was used to detect the expression of p16 gene in paraffin embedded tissues of 54 patients with primary esophageal squamous cell carcinoma. Results The deletion of p16 gene was found in 54 cases of esophageal squamous cell carcinoma (21 cases, 38.9%). The expression of p16 was closely related to tumor grade. The differentiation degree of p16 was significantly lower than that of p16 (P <0.01). p16 gene expression and regional lymph node metastasis also have some relevance. Conclusions The deletion of p16 gene in patients with primary esophageal squamous cell carcinoma is more common than that in patients with primary esophageal squamous cell carcinoma and is closely related to the degree of differentiation of the tumor tissue and lymph node metastasis. Using SABC immunohistochemistry to detect the expression of p16 gene can be used as a supplementary diagnostic tool for primary esophageal squamous cell carcinoma, and it also provides important information for choosing the appropriate treatment plan.