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薄层色谱法,是指将适宜的固定相涂布于玻璃板、塑料或铝基片上,成一均匀薄层。待点样、展开后,与适宜的对照物按同法所得的色谱图作对比,用以进行药物的鉴别、杂质检查或含量测定的方法[1]。本文介绍了薄层层析法的原理,操作方法,展开剂配置,注意事项,应用优点及其在药物鉴别、杂质分析、质量控制等方面的局限性。
Thin-layer chromatography refers to the application of a suitable stationary phase to a glass plate, plastic or aluminum substrate to form a uniform thin layer. To be spotted, unfolded, with the appropriate control by the same method as the chromatogram for comparison, for drug identification, impurity testing or content determination method [1]. This article introduces the principle, operation method, deployment agent configuration, precautions, application advantages and limitations of TLC, including drug identification, impurity analysis, quality control and so on.