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目的探讨蒙古沙鼠全脑缺血再灌注后养血清脑颗粒(Yangxueqingnaokeli,YXQNKL)的治疗作用。方法采用蒙古沙鼠两侧颈总动脉结扎法,缺血30min再灌注5d模型(分为假手术组、缺血再灌注组、养血清脑颗粒治疗组)。用Nissl染色法观察海马CA1区神经元的形态和数量,用免疫组织化学方法观察海马CA1区神经元神经钙离子感应蛋白1(NCS-1)、天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)和谷氨酸盐合成酶(Glusyn)的表达情况。结果与缺血再灌注组比较,缺血再灌注+0.4g/kg养血清脑颗粒治疗组和缺血再灌注+0.8g/kg养血清脑颗粒治疗组Nissl染色显示海马CA1区神经元数量明显增加(P<0.05);免疫组织化学法显示海马CA1区神经元细胞NCS-1、caspase-3和Glusyn的阳性细胞数明显减少(P<0.05)。结论全脑缺血再灌注后,给予养血清脑颗粒能显著增加海马CA1区神经元数量,这与其减少海马CA1区神经元NCS-1、caspase-3和Glusyn的表达有密切的联系。
Objective To investigate the therapeutic effects of Yangxueqingnaokeli (YXQNKL) after cerebral ischemia-reperfusion in Mongolian gerbils. Methods Mongolian gerbils were ligated on both sides of the common carotid artery and were subjected to ischemia for 30 min and then reperfusioned for 5 days (divided into sham operation group, ischemia reperfusion group, Yangxue Qingnao Granule treatment group). Nissl staining was used to observe the morphology and number of neurons in hippocampal CA1 region. Immunohistochemistry was used to observe the neuronal calcium ion-sensing protein 1 (NCS-1) and caspase-3 in hippocampal CA1 region. Expression of caspase-3 and Glusyn. Results Compared with the ischemia-reperfusion group, Nissl staining showed that the number of neurons in hippocampal CA1 region was significantly increased after ischemia-reperfusion +0.4g/kg Yangxueqingnaojing group and ischemia/reperfusion +0.8g/kg Yangxueqingnaojing group. Increased (P<0.05); Immunohistochemistry showed that the number of positive cells of NCS-1, caspase-3, and Glusyn in hippocampal CA1 neurons was significantly reduced (P<0.05). Conclusion After total cerebral ischemic reperfusion, administration of Yangxueqingnao granule can significantly increase the number of neurons in hippocampal CA1 region, which is closely related to the decrease of the expression of NCS-1, caspase-3, and Glusyn in hippocampal CA1 neurons.