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本文报道,用于胞浆内精子注射(ICSI)的,从睾丸活组织标本中采获精子的改良方法,以及对一组病例的对照研究结果。 由非阻塞性无精症睾丸组织中获取精子,主要的困难是标本中存在大量的红细胞,而精子细胞则极其少见。报道一种去除红细胞,从而较易获取精子的方法。 首例个体,取多块睾丸组织,置于含人血清白蛋白2.25％的羟乙基哌嗪乙烷磺酸(HEPES)缓冲Earle液3ml,将睾丸组织剪碎后取出,剩余液体置15ml离心管,300g离心
This article reports an improved method for harvesting sperm from testis tissue specimens for intracytoplasmic sperm injection (ICSI), as well as a controlled study of a group of patients. Acquired sperm from non-obstructive azoospermatoid testis, the main difficulty is the presence of a large number of red blood cells in the specimen, while sperm cells are extremely rare. Report a way to remove red blood cells, which is easier to obtain sperm. The first individual, take a plurality of testicular tissue, placed in 2ml of hydroxyethylpiperazine ethanesulfonic acid (HEPES) containing 2% of human serum albumin buffer Earle liquid 3ml, the testis tissue was cut and removed, the remaining liquid was centrifuged 15ml Tube, 300g centrifuged