应用显色基质、鲎试剂建立起定量检测人血丙种球蛋白（简称Ｉｇ）细菌内毒素的新方法，并应用于５１批Ｉｇ细菌内毒素含量的测定。结果表明，细菌内毒素浓度与相应的吸光度呈良好的线性关系，ｒ＝０．９９８；样品干扰性实验的回收率为９０．２７％；以３．３３Ｅｎ／ｍｌ、６．６７Ｅｎ／ｍｌ分别为５％，１０％Ｉｇ细茵内毒素的最大限量，考察５１批Ｉｇ中的细菌内毒素含量，与家兔热原法结果的符合率为１００％． A new method of quantitative detection of bacterial endotoxin of human gamma globulin (Ig) was established by using chromogenic matrix and 鲎 reagent, and applied to the determination of bacterial endotoxin in 51 batches of Ig. The results showed that the bacterial endotoxin concentration and the corresponding absorbance showed a good linear relationship, r = 0.998; sample interference test recovery was 90.27%; 3.33En / ml, 6.67En / ml were 5% and 10% Ig. The bacterial endotoxin content in 51 batches of Ig was investigated. The coincidence rate was 100% with the result of rabbit pyrogen test.