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本实验采用全细胞电压钳记录方法,研究了内吗啡肽1(EM1)和内吗啡肽2(EM2)对脊髓背角胶状质神经元突触传递的抑制性作用。EM1(1μmol/L)和EM2(1μmol/L)都能够显著抑制微小兴奋性突触后电流(mEPSCs)和微小抑制性突触后电流(mIPSCs)的频率而不改变其幅值。这种抑制作用能被μ受体选择性拮抗剂βfunaltrexamine(βFNA,10μmol/L)阻断。值得注意的是,EM1对mEPSCs和mIPSCs的频率的抑制作用强于EM2。上述结果提示在脊髓胶状质,内吗啡肽通过激活突触前膜上的μ受体,抑制兴奋性和抑制性的突触传递;与EM2相比,EM1可能是脊髓水平的更强效的内源性镇痛剂。
In this study, the inhibitory effect of endomorphin-1 (EM1) and endomorphin-2 (EM2) on the synaptic transmission of neurons in the dorsal horn of spinal cord was investigated using whole-cell voltage-clamp recording method. Both EM1 (1μmol / L) and EM2 (1μmol / L) significantly inhibited the frequencies of mSPS and mIPSCs without changing their amplitude. This inhibitory effect can be μ receptor selective antagonist βfunaltrexamine (βFNA, 10μmol / L) blocked. It is noteworthy that the inhibitory effect of EM1 on the frequency of mEPSCs and mIPSCs is stronger than that of EM2. The above results suggest that in the spinal glias, endomorphin inhibits excitatory and inhibitory synaptic transmission by activating the mu receptor on the presynaptic membrane; EM1 may be more potent at the spinal cord level than EM2 Endogenous analgesics.