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目的:通过对比两种检测流感病毒的方法,寻找一种快速、简便、更适于快速检测的方法,使临床治疗更及时有效。方法:应用碱性磷酸酶抗碱性磷酸酶桥联酶标法(APAAP法)和胶体金免疫层析法(GICA法),检测北京军区总医院呼吸科2007-07/2008-12住院患者中的感染病例共960例。结果:两种方法的一致性检验,甲型流感Kappa=0.765,乙型流感Kappa=0.754,两者均大于0.75,说明两种方法检测甲、乙型流感均有很好的一致性。结论:与APAAP法相比,GICA法快速、简便,更适合于流感快速检测。
OBJECTIVE: To find out a rapid, simple and suitable method for rapid detection by comparing two methods of detecting influenza virus to make the clinical treatment more timely and effective. Methods: Alkaline phosphatase (APAAP) method and colloidal gold immunochromatography (GICA method) were used to detect inpatients with respiratory department of Beijing Military Region General Hospital from July 2007 to December 2008 Of 960 cases of infection. Results: Consistency test between the two methods showed that Kappa = 0.765 and Kappa = 0.754, both of which were greater than 0.75, indicating that the two methods are very consistent in detecting Influenza A and B. Conclusion: Compared with APAAP method, GICA method is rapid and simple, and it is more suitable for rapid detection of influenza.