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目的应用双抗体夹心酶联免疫吸附法(ELISA)检测肺结核患者抗结核治疗前后血高迁移率族蛋白1(HMGB1)水平变化研究,以探讨其临床诊断意义。方法采用ELISA法检测患者血清HMGB1的水平,同时测定活动组患者在抗结核治疗前、治疗后1个月、治疗后3个月及30例好转组的血清HMGB1水平。结果活动组患者比对照组、稳定组的血清HMGB1浓度水平升高,差异具有统计学意义(P<0.05),稳定组患者比对照组的血清HMGB1浓度水平升高,但差异无统计学意义(P>0.05);治疗后1个月、治疗后3个月的血清HMGB1浓度水平比治疗前均有所降低,差异无统计学意义(P>0.05),但其中治疗3个月后有30例活动性肺结核患者病情好转(好转组)比治疗前显著降低,差异具有统计学意义(P<0.05)。结论动态监测肺结核患者血清HMGB1水平,对活动性肺结核的辅助诊断及疗效观察有重要意义。
Objective To investigate the changes of serum high mobility group box 1 (HMGB1) before and after anti-TB therapy in patients with pulmonary tuberculosis by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to explore its clinical significance. Methods Serum levels of HMGB1 were measured by ELISA. The levels of HMGB1 in serum of patients in active group before anti-TB treatment, 1 month after treatment, 3 months after treatment and 30 cases of remission were measured. Results The level of serum HMGB1 in patients in active group was significantly higher than that in control group and stable group (P <0.05). The level of serum HMGB1 in patients in stable group was higher than that in control group, but the difference was not statistically significant (P> 0.05). The levels of serum HMGB1 at 1 month and 3 months after treatment were lower than those before treatment (P> 0.05), but there were 30 cases after 3 months of treatment Patients with active pulmonary tuberculosis improved (improved group) than before treatment was significantly lower, the difference was statistically significant (P <0.05). Conclusion The dynamic monitoring of serum HMGB1 level in patients with pulmonary tuberculosis is of great significance in the diagnosis of active pulmonary tuberculosis and its curative effect.