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目的 探讨乙肝病毒 (HBV)DNA含量与慢性肝病临床病程和乙肝标志物的关系及其在治疗疗效观察中的应用价值。方法 采用TagMan荧光标记探针技术检测 113例各型慢性肝病患者血清HBV -DNA含量。结果 以慢性乙肝轻型HBV -DNA含量最高 ,随肝损害加重 ,HBV -DNA含量逐渐下降。血清HBV -DNA含量在HBeAg阳性组明显高于HBeAg阴性组 ,而与ALT、AST及肝硬化不同Child分级无相关性。治疗前HBV -DNA含量低者疗效好 ,且不同疗效HBV -DNA含量变化不同。结论 定量PCR检测血清HBV -DNA含量对了解慢性乙肝患者病毒复制情况和临床病程的关系及治疗评价有一定价值。
Objective To investigate the relationship between hepatitis B virus (HBV) DNA content and clinical course of chronic liver disease and markers of hepatitis B and its clinical value in the treatment of curative effect. Methods The content of HBV DNA in serum of 113 patients with various types of chronic liver diseases was detected by TagMan fluorescent labeling probe. Results Chronic hepatitis B light HBV-DNA content highest, with increased liver damage, HBV-DNA content decreased. Serum HBV-DNA content in HBeAg-positive group was significantly higher than HBeAg-negative group, but with ALT, AST and cirrhosis of different Child classification no correlation. Before treatment, the effect of low HBV-DNA content is good, and HBV-DNA content varies with different curative effect. Conclusion Quantitative PCR detection of serum HBV-DNA content has a certain value in understanding the relationship between viral replication status and clinical course of patients with chronic hepatitis B and the therapeutic evaluation.