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目的研究碘缺乏、甲状腺功能减退(甲减)对仔鼠海马蛋白激酶C活性的影响,并测定碘缺乏、甲减大鼠仔鼠海马组织c-fos、c-jun的表达,以探讨碘缺乏、甲减调节脑发育的机制。方法分别选用低碘饲料及他巴唑诱导建立低碘及甲减大鼠动物模型,收集生后30 d时仔鼠海马组织,测定海马细胞浆、细胞膜PKC活性。免疫组织化学S-P法染色,观察生后30d时低碘组、甲减组及对照组大鼠仔鼠海马即早基因c-fos、c-jun表达情况并进行图像分析。结果生后30 d低碘组和甲减组仔鼠海马胞液PKC活性略低于对照组,而胞膜PKC活性稍高于对照组,低碘组、甲减组仔鼠海马胞膜PKC活性与胞浆PKC活性比值(1.1871±0.4325,1.4143±0.3940)较对照组(0.6493±0.2943)升高,差异有统计学意义(P<0.01)。生后30d低碘组和甲减组仔鼠海马组织c-fos、c-jun灰度值均显著高于对照组(P<0.01),提示其表达水平明显降低。结论碘缺乏、甲状腺功能减退可影响神经系统及智力发育,可能是低碘、甲减引起海马损害导致学习记忆功能减退的机制之一。
Objective To investigate the effect of iodine deficiency and hypothyroidism (Hypothyroidism) on the activity of protein kinase C in hippocampus of offspring rats, and to determine the expression of c-fos and c-jun in hippocampus of neonatal rats with iodine deficiency and hypothyroidism so as to investigate the effects of iodine deficiency Hypothyroidism regulates brain development. Methods Low iodine diet and methimazole were used to induce the establishment of low iodine and hypothyroidism rat model respectively. The hippocampal tissue of the offspring was collected 30 days after birth and the activity of PKC in hippocampus was measured. Immunohistochemical S-P staining was used to observe the expression of c-fos, c-jun and c-jun in hippocampus of pups in low iodine group, hypothyroidism group and control group at 30 days after birth, and image analysis was performed. Results The PKC activity of hippocampal cytosols in hypodermic and hypothyroidism offspring 30 d after birth was slightly lower than that in control group, but the PKC activity in membrane was slightly higher than that in control group. PKC activity in hippocampus of hypoglycemic group and hypothyroidism group (1.1871 ± 0.4325,1.4143 ± 0.3940) than the control group (0.6493 ± 0.2943), the difference was statistically significant (P <0.01). The gray values of c-fos and c-jun in hippocampus of pups in hypodermic and hypothyroidism groups at 30 days after birth were significantly higher than those in control group (P <0.01), suggesting that the expression levels of c-fos and c-jun were significantly decreased. Conclusions Iodine deficiency and hypothyroidism may affect the nervous system and mental development. It may be one of the mechanisms of hypokalemia and hypothyroidism that lead to impairment of learning and memory in hippocampus.