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背景与目的:EphA1基因是受体酪氨酸激酶家族的成员之一,其过表达可能在恶性肿瘤的发生、发展中起重要的作用。本研究旨在探讨受体酪氨酸激酶EphA1 mRNA在膀胱癌中的表达及其临床意义。方法:采用实时荧光定量PCR法检测50例膀胱癌组织和15例相应癌旁组织中EphA1 mRNA的表达,分析基因表达的差异与临床病理特征间的关系。结果:EphA1 mRNA在膀胱癌中的表达量为106.86±30.40,高于癌旁组织的表达量10.37±2.69,差异有显著性统计学意义(P<0.001);EphA1 mRNA在高级别膀胱癌中的表达量为210.94±50.51,高于低级别膀胱癌组的52.64±16.01(P<0.001),肌层浸润性膀胱癌EphA1 mRNA的表达量为174.69±43.57,显著高于非肌层浸润性膀胱癌的63.14±17.60(P=0.001)。EphA1 mRNA的表达与性别、年龄、肿瘤大小、数目无显著相关性(P>0.05)。结论:EphA1 mRNA的表达在肿瘤组织中明显高于正常组织,且与病理分级、临床分期呈正相关,因此EphA1基因的过表达可能促进膀胱癌的进展。
BACKGROUND & OBJECTIVE: EphA1 gene is a member of the receptor tyrosine kinase family. Its overexpression may play an important role in the occurrence and development of malignant tumors. This study aimed to investigate the expression of receptor tyrosine kinase EphA1 mRNA in bladder cancer and its clinical significance. Methods: The expression of EphA1 mRNA in 50 cases of bladder cancer and 15 cases of paracancerous tissues was detected by real-time fluorescence quantitative PCR, and the relationship between the gene expression and clinicopathological features was analyzed. Results: The expression of EphA1 mRNA in bladder cancer was 106.86 ± 30.40, which was significantly higher than that in paracancerous tissues (10.37 ± 2.69, P <0.001). The expression of EphA1 mRNA in high-grade bladder cancer The expression level of EphA1 mRNA in muscle-invasive bladder cancer was significantly higher than that in non-muscle invasive bladder cancer (210.94 ± 50.51, 52.64 ± 16.01, P <0.001) Of 63.14 ± 17.60 (P = 0.001). There was no significant correlation between EphA1 mRNA expression and sex, age, tumor size and number (P> 0.05). Conclusion: The expression of EphA1 mRNA in tumor tissue is significantly higher than that in normal tissue, and it is positively correlated with pathological grade and clinical stage. Therefore, overexpression of EphA1 gene may promote the progression of bladder cancer.