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选用110只雄性Wistar大鼠,落体致伤法造成大鼠脑损伤模型,放免法检测伤后3小时内4个时限鼠脑挫伤皮质血栓素B_2(TXB_2)与6—酮—前列腺素F_(1a)(6—Keto—PGF_(1a))含量,并通过电镜、光镜检查和伊文思蓝染范围,探讨血栓素A_2(TXA_2)、前列环素(PGI_2)与继发性脑损害的关系以及川芎嗪的作用。结果:挫伤脑组织TXB_2、6—Keto—PGF_(1a)含量在伤后逐渐增高,以3小时增高较著。TXB_2/6—Keto—PGF_(1a)值(T/K值)也逐渐递增;病理检查证实,随伤后时间延长,脑损害程度愈加重。应用川芎嗪治疗后,脑组织TXB_2含量明显降低,T/K值缩小;结果提示:伤后TXA_2和PGI_2这一对微循环调节因子的代谢失衡是加重继发性脑损害的重要因素之一,川芎嗪可对抗TXA_2的生成与活性,减轻TXA_2与PGI_2的代谢失衡,使挫伤脑组织缺血缺氧状态得以部分改善。
A total of 110 male Wistar rats were selected to establish a rat model of brain injury induced by fall injury. Radioimmunoassay was used to detect the levels of cortical thromboxane B_2 (TXB_2) and 6-keto-prostaglandin F_ (1a) (6-Keto-PGF_ (1a)), and to explore the relationship between thromboxane A_2 (TXA_2), prostacyclin (PGI_2) and secondary brain damage by electron microscopy, light microscopy and Evans blue staining. The role of ligustrazine. Results: The contents of TXB_2, 6-Keto-PGF_ (1a) in contusive brain tissue increased gradually after injury and increased significantly at 3 hours. TXB_2 / 6-Keto-PGF_ (1a) value (T / K value) also gradually increased; pathological examination confirmed that with the extension of time after injury, the more the degree of brain damage. After treatment with ligustrazine, the content of TXB_2 in brain tissue decreased and the T / K value decreased. The results suggested that the metabolic imbalance of TXA_2 and PGI_2, which regulate microcirculation, is one of the important factors that aggravate secondary brain damage. Tetramethylpyrazine can antagonize the TXA_2 generation and activity, reduce TXA_2 and PGI_2 metabolic imbalance, so that contusion of brain tissue ischemia and hypoxia can be partially improved.