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目的:分别通过人体和动物实验探讨乳酸阈强度下代谢转变的机制。方法:选取24名体育专业学生作为人体组,进行递增负荷功率自行车运动;选取30只SD大鼠作为大鼠组,进行递增负荷游泳运动。首先确定两组受试各自的乳酸阈强度,后在不吸氧与补充吸氧条件下按相同运动方案进行递增负荷运动至乳酸阈强度,分别于运动前和乳酸阈强度下测定人体组及大鼠组静脉血氧分压、丙酮酸和乳酸含量。结果:(1)人体组和大鼠组在乳酸阈强度下,氧分压平均值分别是33.55±6.49mmHg(不吸氧)、31.86±6.23mmHg(补充吸氧)和58.64±2.01mmHg(不吸氧)、54.82±7.27mmHg(补充吸氧);血乳酸平均值分别是3.61±0.56mmol/L(不吸氧)、3.72±0.58mmol/L(补充吸氧)和5.43±0.55mmol/L(不吸氧)、5.35±0.50mmol/L(补充吸氧)。人体组和大鼠组运动前及乳酸阈强度下,丙酮酸平均值分别是0.97±0.17mmol/L、1.04±0.16mmol/L和0.93±0.25mmol/L、0.91±0.37mmol/L。(2)在乳酸阈强度下,不论是否吸氧,人体组和大鼠组血乳酸含量与氧分压之间均不相关,整个测试过程中人体组血氧饱和度均不低于98%;而二者血乳酸与血丙酮酸含量之间均呈高度显著性差异(P<0.001);二者运动前与乳酸阈强度时的血丙酮酸含量均无显著性差异(P>0.05)。结果表明,运动中由有氧向无氧代谢转变时体内不缺氧,本实验结果在整体水平上支持了糖酵解时丙酮酸转变成乳酸入血,以防止其堆积的观点。
OBJECTIVE: To investigate the mechanism of metabolic transition under the threshold of lactate acid through human and animal experiments respectively. Methods: Twenty-four PE majors were selected as the human body to perform incremental power cycling exercise. Thirty SD rats were selected as the rat group for progressive load swimming exercise. First determine the lactic acid threshold strength of the two groups were tested, and then under the conditions of non-oxygen and oxygen supplementation under the same exercise program for progressive exercise to lactic acid threshold intensity, respectively, before exercise and lactic acid threshold intensity of human body and large Rats venous blood pressure, pyruvate and lactate content. Results: (1) The average oxygen partial pressure of lactate threshold intensity in human and rat groups were 33.55 ± 6.49mmHg, 31.86 ± 6.23mmHg and 58.64 ± 2.01mmHg respectively (Oxygen uptake), 54.82 ± 7.27mmHg (supplemented with oxygen); mean blood lactate were 3.61 ± 0.56mmol / L (not oxygen), 3.72 ± 0.58mmol / L (supplemented with oxygen) and 5.43 ± 0.55mmol / L (Not oxygen), 5.35 ± 0.50mmol / L (oxygen supplementation). The average values of pyruvate before exercise and lactic acid threshold were 0.97 ± 0.17mmol / L, 1.04 ± 0.16mmol / L and 0.93 ± 0.25mmol / L, 0.91 ± 0.37mmol / L respectively in the body and rat groups. (2) In the lactic acid threshold intensity, no matter whether the oxygen is inhaled or not, there is no correlation between the blood lactic acid content and the partial pressure of oxygen in the human body group and the rat group, and the blood oxygen saturation of the human body group is no less than 98% during the whole testing process; There was a highly significant difference between blood lactate and blood pyruvate (P <0.001). There was no significant difference (P> 0.05) in blood pyruvate concentration between the two before exercise and lactic acid threshold. The results show that during exercise from aerobic to anaerobic metabolism changes in the body is not hypoxia, the results of this experiment on the overall level to support the conversion of pyruvate into lactic acid into the blood, in order to prevent the accumulation of point of view.