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本研究通过在大豆基因组数据库中检索拟南芥AtDAO1在大豆中的同源基因,获得了GmDAO1基因序列。通过对GmDAO1基因编码的氨基酸序列及启动子序列进行生物信息学分析,我们发现GmDAO1基因CDS序列全长951bp,编码316个氨基酸。GmDAO1编码的蛋白为亲水性蛋白,具有1个N-糖基化位点、3个激酶磷酸化位点与1个豆蔻酰化位点。结构域分析表明GmDAO1含有双加氧酶与2OG-Fe(II)加氧酶结构域,是2-酮戊二酸依赖性双加氧酶基因(2-ODD)家族的成员。GmDAO1预测的启动子区域含有与激素、胁迫、光应答、生物钟调控和转录因子结合相关的顺式作用元件。系统进化分析结果表明DAO1在豆科植物进化过程中比较保守。组织特异性表达分析结果显示GmDAO1在叶片中表达量最低,在根中表达量最高。因此我们推测其可能参与生长素的代谢途径。
In this study, the GmDAO1 gene sequence was obtained by searching the soybean genome database for the Arabidopsis AtDAO1 homolog in soybean. By bioinformatics analysis of the amino acid sequence and promoter sequence of GmDAO1 gene, we found that CDS sequence of GmDAO1 gene is 951bp in length and encodes 316 amino acids. The GmDAO1-encoded protein is a hydrophilic protein with one N-glycosylation site, three kinase phosphorylation sites and one myristoylation site. Domain analysis revealed that GmDAO1 contains dioxygenase and 2OG-Fe (II) oxygenase domains and is a member of the 2-ketoglutarate-dependent dioxygenase gene (2-ODD) family. The promoter region predicted by GmDAO1 contains cis-acting elements that are involved in hormonal, stress, light response, circadian clock regulation, and transcription factor binding. Phylogenetic analysis showed that DAO1 is more conserved during the evolution of leguminous plants. Tissue-specific expression analysis showed that GmDAO1 expressed the lowest in leaves and highest in roots. Therefore, we speculate that it may participate in the metabolic pathway of auxin.