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利用聚丙烯酰胺凝胶(SDS-PAGE)电泳技术,分析了毒害艾美耳球虫未孢子化卵囊壁可溶性蛋白。用鼠抗毒害艾美耳球虫重组配子体蛋白rEnGAM56和rEnGAM59多克隆抗体,对卵囊壁可溶性蛋白进行了Western blot分析。结果显示,至少有24条较清晰的电泳条带,其中6条为相对明显的主带,其相对分子质量分别为37 000,35 000,34 000,20 000,14 000和12 000。Western blot分析显示,鼠抗rEnGAM59和rEnGAM56多抗均能识别1条条带,前者的相对分子质量约14 000,后者约30 000。推测30 000和14 000卵囊壁蛋白分别来自毒害艾美耳球虫配子体蛋白EnGAM56和EnGAM59。
Polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the protein expression on the unosporified oocysts of Eimeria tenella. Western blot analysis of soluble protein on the oocyst wall was carried out using the rEnGAM56 and rEnGAM59 recombinant mouse gametophyte anti-Eimeria strains. The results showed that there were at least 24 clearer electrophoretic bands, of which 6 belonged to the relatively obvious dominant bands with relative molecular mass of 37 000, 35 000, 34 000, 20 000, 14 000 and 12 000, respectively. Western blot analysis showed that there were 1 band in the anti-rEnGAM59 and rEnGAM56 polyketide. The former had a molecular weight of about 14,000 and the latter about 30,000. It is speculated that 30 000 and 14 000 oocyst wall proteins are derived from poisoning Eimeria gametophytic proteins EnGAM56 and EnGAM59, respectively.