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目的探讨Dusp6在子宫内膜癌中的表达及其与雌激素受体(ER)、孕激素受体(PR)表达的相关性。方法选取南昌县人民医院及南昌大学第二附属医院2014—2015年存档蜡块200例,存档蜡块来自子宫内膜癌患者50例,子宫内膜非典型性增生患者50例,子宫内膜单纯性增生患者50例,正常增生期子宫内膜患者50例。通过免疫组化法(S-P法)检测标本中Dusp6、ER、PR表达水平,分析Dusp6与ER、PR的相关性。结果 Dusp6在正常增生期宫内膜患者中以阳性表达为主,阳性率为96.0%,在子宫内膜单纯性增生患者中阳性率为86.0%,在子宫内膜非典型性增生患者中阳性率为80.0%,在高分化子宫内膜样腺癌中阳性率为60.9%,中分化子宫内膜样腺癌中阳性率为33.3%,低分化子宫内膜样腺癌中阳性率为25.0%。子宫内膜癌患者ER、PR阳性率低于正常增生期子宫内膜、子宫内膜单纯性增生及子宫内膜非典型性增生患者。在子宫内膜癌中Dusp6表达与ER表达呈正相关(r=2.530,P=0.035),与PR表达呈负相关(r=-2.053,P=0.045)。结论 Dusp6表达缺失可能与子宫内膜癌的发病存在相关性,在子宫内膜癌中Dusp6表达与ER表达呈正相关,与PR表达呈负相关。
Objective To investigate the expression of Dusp6 in endometrial carcinoma and its relationship with the expression of estrogen receptor (ER) and progesterone receptor (PR). Methods Two hundred paraffin blocks were archived in Nanchang County People’s Hospital and the Second Affiliated Hospital of Nanchang University from 2014 to 2015. The archived paraffin blocks were from 50 cases of endometrial carcinoma, 50 cases of endometrial atypical hyperplasia, 50 cases of sexual hyperplasia, 50 cases of normal proliferative endometrium. The expression of Dusp6, ER and PR in the specimens was detected by immunohistochemistry (S-P method), and the correlation between Dusp6 and ER and PR was analyzed. Results Dusp6 was mainly expressed in normal proliferative endometrium, the positive rate was 96.0%. The positive rate of Dusp6 in endometrial hyperplasia was 86.0%. The positive rate of Dusp6 in atypical hyperplasia of endometrium The positive rate was 60.9% in well-differentiated endometrioid adenocarcinoma, 33.3% in moderately differentiated endometrioid adenocarcinoma, and 25.0% in poorly differentiated endometrioid adenocarcinoma. The positive rates of ER and PR in patients with endometrial cancer were lower than those in normal proliferative endometrium, endometrial simple hyperplasia and endometrial atypical hyperplasia. In endometrial carcinoma, Dusp6 expression was positively correlated with ER expression (r = 2.530, P = 0.035), and negatively correlated with PR expression (r = -2.053, P = 0.045). Conclusion The loss of Dusp6 expression may be related to the pathogenesis of endometrial cancer. Dusp6 expression is positively correlated with ER expression in endometrial carcinoma and negatively correlated with PR expression.