论文部分内容阅读
Soybean mosaic virus(SMV) could lead to adult-plant system diseases,and cause mottling of soybean seeds.Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624(susceptible) × Dongnong 8143(resistant) and Dongnong 3C628(susceptible) × Tie 6915(resistant).Simple sequence repeat(SSR) markers with bulked segregation analysis(BSA) were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations.The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene.The two dominant genes were identified to be linked on the MLG F by Mendel’s genetics and SSR genetic mapping.The order and distance of markers DPRSMV1 and DSRSMV1 were Sat_229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335.The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133.This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection(MAS).
Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 (Susceptible) × Dongnong 8143 (SMV) resistant) and Dongnong 3C628 susceptible × Tie 6915 (resistant). Simple sequence repeat (SSR) markers with bulked segregation analysis (BSA) were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel’s genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat_229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335.The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM -TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance also in soybean breeding through molecular marker-assisted selection (MAS).