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Our previous study showed that when glutamate receptor(Glu R)6 C terminus-containing peptide conjugated with the human immunodeficiency virus Tat protein(Glu R6)-9c is delivered into hippocampal neurons in a brain ischemic model, the activation of mixed lineage kinase 3(MLK3) and c-Jun NH2-terminal kinase(JNK) is inhibited via Glu R6-postsynaptic density protein 95(PSD95). In the present study, we investigated whether the recombinant adenovirus(Ad) carrying Glu R6 c could suppress the assembly of the Glu R6-PSD95-MLK3 signaling module and decrease neuronal cell death induced by kainate in hippocampal CA1 subregion. A seizure model in Sprague-Dawley rats was induced by intraperitoneal injections of kainate. The effect of AdGlur6-9c on the phosphorylation of JNK, MLK3 and mitogen-activated kinase kinase 7(MKK7) was observed with western immunoblots and immunohistochemistry. Our findings revealed that overexpression of Glu R6 c inhibited the interaction of Glu R6 with PSD95 and prevented the kainate-induced activation of JNK, MLK3 and MKK7. Furthermore, kainate-mediated neuronal cell death was significantly suppressed by Glu R6 c. Taken together, Glu R6 may play a pivotal role in neuronal cell death.
Our previous study showed that when glutamate receptor (Glu R) 6 C terminus-containing peptide conjugated with the human immunodeficiency virus Tat protein (Glu R6) -9c is delivered into hippocampal neurons in a brain ischemic model, the activation of mixed lineage kinase 3 (MLK3) and c-Jun NH2-terminal kinase (JNK) are inhibited via Glu R6-postsynaptic density protein 95 (PSD95). In the present study, we investigated whether the recombinant adenovirus (Ad) carrying Glu R6 c could suppress the assembly of the Glu R6-PSD95-MLK3 signaling module and decreasing neuronal cell death induced by kainate in hippocampal CA1 subregion. A seizure model in Sprague-Dawley rats was induced by intraperitoneal injections of kainate. The effect of AdGlur 6-9c on the phosphorylation of JNK , MLK3 and mitogen-activated kinase kinase 7 (MKK7) was observed with western immunoblots and immunohistochemistry. Our findings revealed that overexpression of Glu R6 c inhibited the interaction of Glu R6 with PSD95 and prevented the kainate-induced activation of JNK, MLK3 and MKK7. Furthermore, kainate-mediated neuronal cell death was significantly suppressed by Glu R6 c. Taken together, Glu R6 may play a pivotal role in neuronal cell death.