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目的:研究IFITM1基因转染对子宫颈癌HeLa细胞的影响,以及抑制子宫颈癌HeLa细胞生长的作用。方法:应用脂质体法介导IFITM1基因转染子宫颈HeLa细胞,用RT-PCR方法、免疫荧光法、MTT法及流式细胞术检测IFITM1基因转染HeLa细胞的生物学特性。结果:转染后的HeLa细胞:①RT-PCR检测及激光共聚焦显示重组质粒组中mRNA及蛋白表达含量明显高于对照组及空载体组,而对照组及空载体组蛋白表达无统计学差异。②MTT结果显示重组质粒组细胞存活率均明显低于空载体组及未转染HeLa细胞组,而后两组无统计学差异。③AnnexinV/PI双色流式细胞仪检测表明,重组质粒组的HeLa细胞凋亡水平明显高于空载体组和未转染HeLa细胞组,有统计学差异。结论:IFITM1蛋白与子宫颈鳞癌有着密切的关系,该基因可能是一种潜在的抑制子宫颈鳞癌发生的基因。
Objective: To investigate the effect of IFITM1 gene transfection on cervical cancer HeLa cells and the effect of inhibiting the growth of cervical cancer HeLa cells. Methods: HeLa cells were transfected with IFITM1 gene by liposome method. The biological characteristics of HeLa cells transfected with IFITM1 gene were detected by RT-PCR, immunofluorescence, MTT and flow cytometry. Results: Transfection of HeLa cells: ①RT-PCR detection and confocal laser scanning showed that the recombinant plasmid group mRNA and protein expression was significantly higher than the control group and empty vector group, while the control group and empty vector histological expression was not statistically different . ② MTT results showed that the survival rate of recombinant plasmid group were significantly lower than the empty vector group and non-transfected HeLa cell group, and then there was no significant difference between the two groups. ③AnnexinV / PI two-color flow cytometry showed that the recombinant plasmids HeLa cell apoptosis levels were significantly higher than the empty vector group and untransfected HeLa cell group, with statistical differences. Conclusion: IFITM1 protein has a close relationship with cervical squamous cell carcinoma, which may be a potential gene that inhibits the occurrence of cervical squamous cell carcinoma.