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目的:本课题旨在利用现代生物模型,验证中药方剂对骨折愈合的影响,为中医接骨理论提供科学依据和筛选新型方剂。方法:根据中医骨伤的组方原则,本研究从抗炎镇痛,活血化瘀和接骨续筋3类中药中各选出2味最常用的中药,采用均匀设计法将6味中药按照不同剂量配比,组成5个方剂。选择炎症因子(NO)释放、人体静脉上皮细胞(HUVEC-12)和成骨细胞(UMR-106)增殖试验分别反映抗炎镇痛,活血化瘀和接骨续筋的功效,测试各方剂对骨折愈合的影响。结果:5组复方体外试验均表现出单重或多重促进骨折愈合的作用。其中5号复方(大黄30 g,栀子、三七、红花各9 g,接骨木30 g,续断15 g)显示出全面的抑制NO释放,促进HUVEC-12和UMR-106增殖的活性。结论:证明了中药复方能从多个方面加速骨折愈合,其主效应包括抗炎镇痛,促进骨折局部的血液循环和新骨再生。提供了以大黄、栀子为解毒君药,以三七、红花活血化瘀作臣药,续断、接骨木为佐使的基本外敷复方,实际应用时可根据辨证观察作适当增减,达到最佳疗效。
Objective: The purpose of this project is to use the modern biological model to verify the effect of traditional Chinese medicine prescriptions on fracture healing and provide a scientific basis and screening new prescriptions for TCM osteoporosis theory. Methods: According to the prescription principle of TCM bone injury, two kinds of traditional Chinese medicine, the two most commonly used herbs, were selected from three kinds of traditional Chinese medicines of anti-inflammatory and analgesic, blood-activating and blood- Dose ratio, composed of 5 prescriptions. Select the release of inflammatory factors (NO), human vein epithelial cells (HUVEC-12) and osteoblasts (UMR-106) proliferation test respectively reflect the efficacy of anti-inflammatory analgesic, promoting blood circulation and bone continued tendon, Healing effects. Results: The five groups of compound in vitro tests showed the role of single or multiple to promote fracture healing. The compound 5 (rhubarb 30 g, gardenia, notoginseng, safflower 9 g, elderberry 30 g, continued broken 15 g) showed a comprehensive inhibition of NO release, and promote the proliferation of HUVEC-12 and UMR-106 activity . Conclusion: It is proved that traditional Chinese medicine compound can accelerate fracture healing in many aspects. Its main effects include anti-inflammatory and analgesic, promoting blood circulation and new bone regeneration. Provided with rhubarb, gardenia as the antidote kingdoms, notoginseng, safflower blood circulation for Chen medicine, continued off, elderberry as the basic external application of compound, the actual application of dialectical observation may be appropriate to increase or decrease, To achieve the best effect.