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目的:建立HPLC测定大鼠血浆和组织中阿特拉津(ATR)及其主要代谢物脱乙基脱异丙基阿特拉津(DEDIA)的浓度,研究ATR及DEDIA在大鼠组织的分布特征。方法:大鼠单剂量灌胃ATR,分别在给药后0.5,2,6和12 h取血和组织样本,以乙腈沉淀蛋白,0.5%冰醋酸甲醇液-0.5%冰醋酸水溶液梯度洗脱,HPLC测定样品浓度。结果:ATR在体内被快速且大量的代谢为DEDIA,血浆和组织中均能检测到ATR和DEDIA,尤以DEDIA为主;DEDIA在各组织中广泛分布,但消除速率不一,以肝、肾消除速度最慢。结论:ATR及DEDIA在大鼠各组织中的分布及消除规律,可为临床毒代动力学提供参考。
OBJECTIVE: To establish a HPLC method for the determination of the concentration of atrazine (ATR) and its major metabolite dedethylazide (ATRA) in rat plasma and tissues and to study the distribution of ATR and DEDIA in rat tissues feature. Methods: Rats were given single dose of ATR, and blood and tissue samples were taken at 0.5, 2, 6 and 12 h after administration. The samples were precipitated with acetonitrile, 0.5% glacial acetic acid and 0.5% glacial acetic acid. Determination of sample concentration by HPLC. Results: ATR was rapidly and massively metabolized into DEDIA in vivo, and ATR and DEDIA were detected in plasma and tissues, especially in DEDIA. DEDIA was widely distributed in various tissues but with different elimination rates, with liver and kidney Eliminate the slowest. Conclusion: The distribution and elimination of ATR and DEDIA in various tissues of rats may provide reference for clinical toxicokinetics.