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采用邻苯二甲醛柱前衍生,反相高效液相色谱、萤光检测和单泵洗脱分析测定牡蛎提取物中的牛磺酸。分析方法为在2-巯基乙醇存在下.与OPA衍生反应后,在ODS柱上分离。本方法回收率较好,灵敏度高.对照品溶液测试在贮藏条件下稳定。
O-phthalaldehyde precolumn derivatization, RP-HPLC, fluorescence detection, and single pump elution analysis were used to determine taurine in oyster extracts. The analytical method is in the presence of 2-mercaptoethanol. After derivatization with OPA, it was separated on an ODS column. The method has better recovery and high sensitivity. The reference solution test is stable under storage conditions.