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目的:建立简便准确的固相萃取-高效液相色谱法测定藿香正气口服液中厚朴酚与和厚朴酚的含量。方法:选用C18键合硅胶固相萃取小柱对藿香正气口服液进行预处理,分别以水和甲醇为淋洗剂和洗脱剂,收集甲醇洗脱物用于HPLC分析。采用Scienhome-Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水-甲酸(75∶25∶0.25)为流动相,流速为1.0 mL·min-1,检测波长为294nm。结果:方法除杂效果明显,重复性的RSD小于1.0%,厚朴酚与和厚朴酚的平均回收率分别为96.7%和97.2%。结论:本方法操作简便快速,环境友好,结果准确可靠,适用于藿香正气口服液中厚朴酚与和厚朴酚的含量测定。
Objective: To establish a simple and accurate solid phase extraction - HPLC method for the determination of honokiol and oral honokiol and honokiol content. Methods: C18 bonded silica SPE column was used to pretreat Huoxiangzhengqi Oral Liquid, and eluted with water and methanol respectively. The methanol eluate was collected for HPLC analysis. The mobile phase was methanol-water-formic acid (75:25:0.25) at a flow rate of 1.0 mL · min-1 using a Scienhome-Kromasil C18 column (4.6 mm × 250 mm, 5 μm) with a detection wavelength of 294 nm. Results: The method showed obvious effect of removing impurities and the RSD of repeatability was less than 1.0%. The average recoveries of honokiol and honokiol were 96.7% and 97.2%, respectively. Conclusion: The method is simple and rapid, environment-friendly and the results are accurate and reliable. It is suitable for the determination of honokiol and honokiol in Huoxiangzhengqi Oral Liquid.