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采用CCK-8细胞增殖检测、流式细胞仪、Hoechst 33258染色、细胞周期检测、细胞凋亡检测等研究了二氢青蒿素对人成骨肉瘤MG-63细胞凋亡的诱导作用。细胞增殖检测发现二氢青蒿素对MG-63细胞48h及72h时的50%抑制率分别为44.87μmol/L和19.15μmol/L;细胞周期检测发现细胞生长被阻滞在G_0/G_1期;Hoechst33258染色凋亡检测发现细胞核出现致密的固缩形态及颗粒状荧光;Annexin V-FITC双染细胞凋亡检测发现二氢青蒿素对MG-63细胞的凋亡率为45.10%。表明:二氢青蒿素可明显诱导MG-63细胞的凋亡,并且MG-63细胞的凋亡与二氢青蒿素存在剂量与时间依赖关系,二氢青蒿素对人成骨肉瘤MG-63细胞凋亡的诱导具有显著作用,这将为进一步研究细胞凋亡机制提供基础和实验依据。
The effects of dihydroartemisinin on the apoptosis of human osteosarcoma MG-63 cells were studied by CCK-8 cell proliferation assay, flow cytometry, Hoechst 33258 staining, cell cycle assay and apoptosis assay. Cell proliferation assay showed that the 50% inhibition rates of dihydroartemisinin to MG-63 cells at 48h and 72h were 44.87μmol / L and 19.15μmol / L, respectively. Cell cycle arrest showed that cell growth was arrested in G_0 / G_1 phase; Hoechst33258 staining showed that the cells were densely condensed and granular pyknosis. Annexin V-FITC staining showed that the apoptotic rate of MG-63 cells was 45.10%. The results showed that dihydroartemisinin could induce apoptosis of MG-63 cells, and the apoptosis of MG-63 cells was dose-dependent and time-dependent. The effect of dihydroartemisinin on osteosarcoma MG -63 cell apoptosis induction has a significant role, which will provide the basis and experimental evidence for further study of apoptosis mechanisms.