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目的探讨巴曲酶对大鼠脑缺血再灌注FasmRNA和FasLmRNA表达变化的影响。方法取健康Wister大鼠40只,随机分5组,即Ⅰ组为假手术组,Ⅱa组为等渗盐水缺血6h组,Ⅱb为等渗盐水缺血6h再灌注6h组,Ⅲa组巴曲酶缺血6h组,Ⅲb组巴曲酶缺血6h再灌注6h组。线栓法建立大鼠大脑中动脉闭塞(MCAO)及再通模型,应用RT-PCR技术检测MCAO及再灌注后缺血半暗带皮质FasmRNA和FasLmRNA表达水平。结果Ⅰ组Fas mRNA、FasLmRNA均未见表达,Ⅱa组表达水平明显增高(分别是0.90±0.05,0.45±0.02),Ⅱb表达水平明显增高(分别为0.94±0.06和0.51±0.03),Ⅲa组表达水平明显下降(分别是0.45±0.03,0.40±0.06),Ⅲb组表达水平明显下降(分别是0.22±0.03,0.16±0.01)。结论巴曲酶可影响脑缺血再灌注FasmRNA和FasLmRNA表达水平。
Objective To investigate the effect of batroxobin on the expression of FasmRNA and FasL mRNA in rats with cerebral ischemia-reperfusion injury. Methods Forty healthy Wister rats were randomly divided into 5 groups: group Ⅰ was sham operation group, group Ⅱa was isotonic saline ischemia 6h group, group Ⅱb was isotonic saline ischemia 6h reperfusion 6h group, group Ⅲ a 6h ischemia group, 6h ischemia group and 6h ischemia group. The middle cerebral artery occlusion (MCAO) and the recanalization model were established by the suture method. The expression of Fas mRNA and FasL mRNA in the ischemic penumbra cortex were detected by RT-PCR. Results The expressions of Fas mRNA and FasL mRNA were not observed in group Ⅰ, the expressions in group Ⅱa were significantly higher (0.90 ± 0.05 and 0.45 ± 0.02 respectively), the expression of Ⅱb was significantly higher (0.94 ± 0.06 and 0.51 ± 0.03 respectively) (0.45 ± 0.03,0.40 ± 0.06, respectively), while the expression level in group Ⅲb decreased significantly (0.22 ± 0.03,0.16 ± 0.01, respectively). Conclusion Batroxobin can influence the expression of Fas mRNA and FasL mRNA in cerebral ischemia / reperfusion rats.