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1目的 探讨女阴及子宫肿瘤组织人乳头瘤病毒 (HPV) 16 / 18DNA感染及其意义。2方法 应用原位杂交技术对 3个传代的细胞系、96例宫颈标本、75例子宫内膜标本及 33例女阴尖锐湿疣标本 HPV 16 / 18DNA进行了检测。3结果 尖锐湿疣组织 HPV16 / 18DNA感染阳性表达 3例 (9.1% ) ,正常宫颈 2例 (2 0 .0 % )、子宫颈上皮肉瘤变 (CIN ) 5例 (2 7.8% )、CIN 9例 (39.1% )、宫颈鳞癌 2 7例 (6 0 .0 % ) ,4组之间比较差异有极显著性 (χ2 =7.92 3,P<0 .0 1)。2 7例正常子宫内膜组织 HPV16 / 18DNA表达均为阴性 ,48例子宫内膜癌 3例呈阳性 (6 .2 % ) ,其中 1例为腺鳞癌 ,1例为透明细胞癌。4结论 生殖道上皮中存在多种 HPV亚型混合感染 ,子宫内膜癌组织中可见HPV16 / 18感染。
1 Objective To investigate the HPV16 / 18 DNA in female and uterine tumor tissue and its significance. 2 Methods In situ hybridization was used to detect HPV16 / 18 DNA in three passaged cell lines, 96 cervical specimens, 75 endometrial specimens and 33 female genital warts. Results The positive rate of HPV16 / 18 DNA in condyloma acuminata tissue was 3 cases (9.1%), 2 cases (20.0%) in normal cervix, 5 cases (2 7.8%) in CIN, 9 cases 39.1%) and 27 cases of cervical squamous cell carcinoma (60.0%). There was significant difference between the 4 groups (χ2 = 7.92 3, P <0.01). The expression of HPV16 / 18 DNA was negative in 27 cases of normal endometrium and 3 of 48 cases of endometrial carcinoma (6.2%), of which 1 was adenosquamous carcinoma and 1 was clear cell carcinoma. 4 Conclusion Multiple genital HPV subtypes are present in the reproductive tract epithelium. Infection with HPV16 / 18 is seen in endometrial cancer.