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老龄化可引起铁元素在脑内的蓄积。随着年龄的增加,脉络丛上皮可能是脑铁毒性、炎症反应和氧化应激损伤的重要结构。本文旨在研究铁代谢调节中的关键激素——铁调素在脉络丛上皮表达的年龄变化,及其与白细胞介素-6(interleukin-6,IL-6)和信号转导子及转录激活子3(signal transducer and activator of transcription 3,Stat3)的关系。本文以不同年龄段(3、6、9、12、15、18、21、24、27、30、33和36月龄)大鼠为研究对象,用实时定量RT-PCR和Western blot法检测铁调素及IL-6/Stat3信号途径相关蛋白在脉络丛上皮的表达变化。结果显示,3~18月龄年轻大鼠脉络丛上皮铁调素m RNA没有明显变化,21~36月龄大鼠的铁调素m RNA水平显著增加。脉络丛上皮IL-6和p-Stat3 m RNA和蛋白表达的年龄变化趋势与铁调素m RNA一致。以上结果提示,随着年龄的增加,炎症因子IL-6可能作用于相应的受体,通过Stat3信号转导途径,参与调节铁调素在脉络丛上皮的表达变化。
Aging can cause the accumulation of iron in the brain. With age, choroid plexus may be an important structure of brain iron toxicity, inflammation and oxidative stress injury. This study aimed to investigate the age-related changes in the expression of hepcidin in the choroid plexus and its relationship with interleukin-6 (IL-6), signal transducers and transcriptional activation 3 (signal transducer and activator of transcription 3, Stat3). In this paper, different age groups (3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33 and 36 months old) as the research object, using real-time quantitative RT-PCR and Western blot detection of iron The expression of IL-6 / Stat3 signal pathway proteins in choroid plexus epithelium. The results showed that there was no significant change of hepcidin m RNA in the choroid plexus of young rats aged 3 ~ 18 months, and hepcidin m RNA level in 21 ~ 36 months old rats increased significantly. The age trend of IL-6 and p-Stat3 m RNA and protein expression in choroid plexus is consistent with that of hepcidin m RNA. The above results suggest that IL-6, an inflammatory cytokine, may act on the corresponding receptors and may be involved in the regulation of hepcidin expression in the choroid plexus through the Stat3 signaling pathway.