聚合酶链反应(PCR)又称无细胞分子克隆系统或体外酶促基因扩增法,是由Cetus公司Kary B Mullis和加利福尼亚大学Henery A、Erlich于1985年联合创造的。该技术可将极微量的靶DNA特异性地扩增上百万倍,从而大大提高了对DNA分子的分析和检测能力〔1〕。自PCR技术创建以来,在广大科? Polymerase chain reaction (PCR), also known as cell-free molecular cloning system or in vitro enzymatic gene amplification, was co-created by Cetus, Kary B Mullis and the University of California, Henery A, Erlich in 1985. The technology can be a very small amount of target DNA specific amplification millions of times, thus greatly improving the analysis and detection of DNA molecules [1]. Since the creation of PCR technology, in the majority of subjects?