目的比较不同的方法对白假丝酵母菌体外生物膜药敏性检测的差异。方法分别采用菌落计数法(CFU)、AlamarBlue试剂法、XTT减低法、MTT法对白假丝酵母菌体外48h成熟生物膜的药敏性进行检测,并将AlamarBlue试剂法、XTT减低法、MTT法与CFU法进行比较,观察其相关性及差异性。结果 AlamarBlue试剂法、XTT减低法、MTT法与CFU法都有较高的相关性,相关系数分别为r=0.969、r=0.971、r=0.982(P<0.01);与CFU法的差异分别为(0.093±0.127)、(0.054±0.113)、(0.013±0.066),其差异无统计学意义(P>0.05)。结论 AlamarBlue试剂法、XTT减低法、MTT法均可替代CFU法对白假丝酵母菌生物膜进行药敏检测。MTT法与CFU法的相关性最大,差异性最小;AlamarBlue试剂作为一种新的试剂,操作简单,对细胞及人类无毒害,更加适合高通量检测。 Objective To compare the differences of drug sensitivity between Candida albicans and different biofilms by different methods. Methods The susceptibility of Candida albicans to 48 h mature biofilm was detected by colony counting method (CFU), AlamarBlue reagent method, XTT reduction method and MTT method respectively. The effects of AlamarBlue reagent method, XTT reduction method and MTT method Compared with the CFU method to observe the correlation and differences. Results There was a high correlation between AlamarBlue reagent method, XTT reduction method and MTT method and CFU method. The correlation coefficients were respectively r = 0.969, r = 0.971, r = 0.982 (P <0.01) (0.093 ± 0.127), (0.054 ± 0.113) and (0.013 ± 0.066), respectively, with no significant difference (P> 0.05). Conclusion AlamarBlue reagent method, XTT reduction method, MTT method can replace CFU method for Candida albicans biofilm susceptibility testing. The correlation between MTT method and CFU method is the largest and the difference is the smallest. AlamarBlue reagent, as a new reagent, is simple in operation and non-toxic to cells and humans and is more suitable for high-throughput detection.