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通过灵芝发酵液酸性醇提物对小鼠的镇咳、祛痰实验,及烟熏小鼠肺部组织的形态学观察,研究灵芝酸性醇提物抗慢性支气管炎的疗效。灵芝发酵全液,不同剂量的灵芝酸乙醇提取物、灵芝酸正丁醇提取物均能显著延长二氧化硫诱发的小鼠咳嗽潜伏期,并显著抑制小鼠咳嗽次数,有明显的镇咳作用。60mg/kg给药量的正丁醇提取物使小鼠气道酚红排泄量较对照组提高了50.7%,祛痰疗效也明显优于双黄连口服液。肺组织切片病理检查表明,灵芝酸性提取物明显减轻烟熏小鼠的气管、支气管粘膜损伤,慢性支气管炎的组织病理学变化明显轻于单纯烟熏模型组,且抗炎、抗损伤作用与双黄连相当,这可为灵芝酸产品的研究开发提供一定的药理依据。
Through the anti-tussive and expectorant experiments of mice with the alcoholic extract of Ganoderma lucidum fermentative liquid, and the morphological observation of the lung tissue of smoked mice, the curative effect of Ganoderma lucidum acid alcohol extract against chronic bronchitis was studied. Whole liquid Ganoderma lucidum fermentation, different doses of ethanol extract of Ganoderma lucidum, n-butanol extract of Ganoderma lucidum can significantly prolong the incubation period of cough induced by sulfur dioxide in mice, and significantly inhibited the number of coughs in mice, and had significant antitussive effect. The n-butanol extract with 60mg/kg dose increased the airway phenol red excretion in mice by 50.7% compared with the control group. The efficacy of sputum was also significantly better than that of Shuanghuanglian Oral Liquid. Pathological examination of lung tissue sections showed that Ganoderma lucidum acid extract significantly reduced tracheobronchial mucosal damage in smoked mice, and the histopathological changes of chronic bronchitis were significantly lighter than those in the pure smoked model group, and the anti-inflammatory and anti-injury effects were doubled. Coptis, which can provide a certain pharmacological basis for the research and development of Ganoderma acid products.