目的:分别以照射前的人鼻咽鳞癌CNE-1细胞和高剂量X射线(50Gy)照射后的CNE-1细胞为研究对象,探讨高剂量X射线照射后的CNE-1细胞的某些生物学特性的变化。方法:采用细胞毒实验(SRB法)检测两组细胞在化疗药物作用下的IC50值,RT-PCR测两组细胞MDR-1基因,共聚焦显微镜测P-gp蛋白,对比两组细胞的耐药性;MTT法测定两组细胞生长曲线,共聚焦显微镜检测Ki-67蛋白,比较两组细胞的生长增殖能力;采用细胞黏附实验比较两组细胞的黏附性,RT-PCR检测两组细胞PECAM-1基因的表达,探讨PECAM-1和细胞黏附的相关性。结果:照射后CNE-1细胞在多柔比星作用下的IC50值是照射前细胞的1.8倍(P=0.004),在紫杉醇作用下的IC50值是照射前细胞的5.3倍(P=0.003),两组细胞MDR-1基因半定量值分别为0.17±0.01和0.34±0.04(P=0.002),照射后细胞P-gp蛋白高表达于照射前细胞;在对数生长期时,照射后细胞生长快于照射前细胞,且其Ki-67蛋白表达高于照射前细胞;照射后细胞在各时间点的黏附率都明显高于照射前细胞,两组数值比较差异有统计学意义,P<0.05;照射前后两组细胞PECAM-1基因的半定量值分别为0.40±0.02和0.51±0.01,P=0.002。结论:高剂量X射线照射后的CNE-1细胞产生耐药性;生长增殖能力及黏附特性高于照射前细胞,PECAM-1基因也高表达,Ki-67及PECAM-1均可能参与耐药。 OBJECTIVE: To investigate the effects of high dose X-ray irradiation on CNE-1 cells and CNE-1 cells exposed to high dose of X-rays (50Gy) Changes in biological characteristics. Methods: The cytotoxicity test (SRB method) was used to detect the IC50 values ​​of the two groups of cells under the action of chemotherapeutic drugs. The MDR-1 gene expression in both groups was measured by RT-PCR. The expression of P-gp protein was detected by confocal microscopy. The cell growth curve was measured by MTT assay. Ki-67 protein was detected by confocal microscopy and the cell proliferation was compared between the two groups. The adhesion of the two groups of cells was evaluated by cell adhesion assay. The expression of PECAM -1 gene expression, explore the correlation between PECAM-1 and cell adhesion. Results: The IC50 of CNE-1 cells after irradiation was 1.8 times higher than that before irradiation (P = 0.004), and the IC50 value of paclitaxel was 5.3 times higher than that before irradiation (P = 0.003) The semi-quantitative value of MDR-1 gene in two groups of cells was 0.17 ± 0.01 and 0.34 ± 0.04 (P = 0.002) respectively. The expression of P-gp protein was significantly higher in pre-irradiated cells than in irradiated cells. In the logarithmic growth phase, The growth rate was faster than that before irradiation, and Ki-67 protein expression was higher than that before irradiation; the adhesion rate of cells at each time point after irradiation was significantly higher than that before irradiation, the difference between the two groups was statistically significant, P < 0.05. The semi-quantitative values ​​of PECAM-1 gene in two groups before and after irradiation were 0.40 ± 0.02 and 0.51 ± 0.01, respectively, P = 0.002. CONCLUSION: The CNE-1 cells after high-dose X-ray irradiation have drug resistance, their growth, proliferation and adhesion properties are higher than those before irradiation, and the expression of PECAM-1 gene is also high. Ki-67 and PECAM-1 may be involved in drug resistance .