目的 :观察明党参多糖对转录活化核蛋白因子NF -κB(nuclearfactorofKappaB)结合活性的影响。方法 :在内毒素 (LPS)诱导J744 .a.1小鼠的巨噬细胞株 ,可诱生出较高的NF -κB活性 ,分别采用LPS先刺激、LPS后刺激和LPS与明党参多糖同时刺激等 3种方法对其处理 ,并分离核蛋白 ,行聚丙酰凝胶电泳及光密度扫描定量分析。结果 :发现明党参多糖在 3种情况下均可降低LPS刺激所致的NF -κB结合活性。结论 :明党参多糖可显著降低LPS刺激所致的NF -κB的结合活性 Objective : To observe the effect of polysaccharides of Radix Codonopsis on the binding activity of nuclear factor of NF-κB (KappaB). METHODS: Macrophages from J744.a.1 mice induced by endotoxin (LPS) induced higher NF-κB activity, stimulated by LPS, stimulated by LPS, stimulated by LPS and stimulated by polysaccharide Three methods were used to treat them, and the nuclear proteins were separated and subjected to polyacrylamide gel electrophoresis and quantitative analysis of optical density scanning. RESULTS: It was found that the polysaccharides of D. candidum could reduce the NF-kappaB binding activity induced by LPS stimulation under three conditions. Conclusion : The polysaccharide of radix pilosulae can significantly reduce the binding activity of NF-κB induced by LPS stimulation