论文部分内容阅读
目的:了解卡介苗对人外周血单个核细胞(hPB-MC)TLR4的表达调节,以及BCG介导免疫细胞活化效应的新机制。方法:研究BCG对hPBMC的TLR4表达的调节,以及对表达TLR4的淋巴细胞的免疫活化效应,以(hPBMC+PBS)作为空白对照组,应用流式细胞仪对TLR4进行检测,ELISA方法测定BCG刺激组与对照组的IFN-γ和TNF-α的表达。结果:经过BCG刺激后,TLR4表达大大增加(P<0.01),且随时间增加而增强,在72 h时,BCG组的TLR4表达率为(44.73±0.0066)%,而对照组的表达率仅为(1.02±0.0024)%。BCG可以促进淋巴细胞增殖,且这种增强作用也存在一定时间依赖性,在BCG与hPBMC共同孵育24 h、48h和72 h后,BCG组IFN-γ和TNF-α的表达量显著高于对照组(PBS组),差异有统计学意义(P<0.05),且这种增强作用存在一定的时间依赖性。结论:BCG对hPBMC的TLR4表达有正调节作用,并加强表达TLR4的hPBMC的免疫活化作用。
OBJECTIVE: To investigate the regulation of TLR4 expression on human peripheral blood mononuclear cells (hPB-MC) and the new mechanism of BCG-mediated activation of immune cells. Methods: The regulation of TLR4 expression by BCG on hPBMC and the immune activation of TLR4-expressing lymphocytes were studied. (HPBMC + PBS) was used as a blank control group, TLR4 was detected by flow cytometry, and BCG stimulation Group and control group of IFN-γ and TNF-α expression. Results: The expression of TLR4 in BCG group was significantly increased (P <0.01) after BCG stimulation, and increased with time. At 72 h, the expression of TLR4 in BCG group was (44.73 ± 0.0066)%, while the expression rate of TLR4 in control group was (1.02 ± 0.0024)%. BCG can promote lymphocyte proliferation, and this enhancement also has a time-dependent manner. After BCG incubated with hPBMC for 24 h, 48 h and 72 h, the expression of IFN-γ and TNF-α in BCG group was significantly higher than that in control Group (PBS group), the difference was statistically significant (P <0.05), and this enhancement there is a certain time-dependent. CONCLUSION: BCG has a positive regulatory effect on TLR4 expression in hPBMC and enhances the activation of hPBMC expressing TLR4.