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目的：通过监测、分析多药耐药菌感染的临床分布情况，为有效的预防控制多药耐药菌感染提供参考依据。方法：采用前瞻性调查方法对我院2014年1月—2014年12月临床标本中检出的104株多药耐药菌进行目标性监测，常规分离培养并鉴定菌种，根据标准筛选多药耐药菌，并对多药耐药菌的分布情况进行分析。结果：检出的104株多药耐药菌前5位菌株依次为屎肠球菌、凝固酶阴性葡萄球菌、大肠埃希菌、铜绿假单胞菌、鲍曼不动杆菌，分别占31.73%、20.19%、10.57%、7.69%、7.69%；标本来源主要为尿液、痰液、分泌物，分别占43.27%、27.88%、18.27%；尿液中多药耐药菌以屎肠球菌为主，痰液中多药耐药菌以铜绿假单胞菌、鲍曼不动杆菌为主，分泌物中多药耐药菌以凝固酶阴性葡萄球菌为主；各临床科室均有检出多药耐药菌，检出科室前3位依次为神外科、神内科、心内科。结论：通过多药耐药菌临床分布风险评估确定预防控制重点，加强对高危科室、高危环节和易感人群的干预，减少多药耐药菌感染和传播。“,”Objective Through monitoring and analyzing the clinical distribution of multidrug resistant bacteria infection, effective prevention and control of multidrug resistant bacteria infection to provide reference.Methods With prospective method for target monitoring in our hospital from 2014 January to 2014 December was detected in clinical specimens of 104 strains of multidrug resistant bacteria,then conventional isolation and identification of bacteria,multidrug resistant bacteria were screened out according to the standard,and the distribution of multidrug resistant bacteria analysis.Resutle A total of 104 strains of multidrug resistant bacteria before 5 strains were Enterococcus faecium, coagulase negative staphylococcus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter Bauman,accounted for 31.73%, 20.19%, 10.57%, 7.69%, 7.69%; was mainly from urine, sputum specimens, secretions, accounted for 43.27%, 27.88%, 18.27%;multidrug resistant bacteria in the urine of Enterococcus faecium based,multidrug resistant bacteria in sputum in Pseudomonas aeruginosa, Acinetobacter Bauman based ,multidrug resistant bacteria in secretion in coagulase negative staphylococci based; clinical departments were detected by multidrug resistant bacteria,the detection department before 3 were Department of Neurosurgery ,Department of Neurology, Internal Medicine-Cardiovascular Department.Conclusion Through the multidrug resistant bacteria in clinical risk assessment to determine the distribution of control and prevention in high-risk departments, focus, strengthen high-risk areas and susceptible population intervention, reduce the infection and spread of multi drug resistant bacteria.